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リコンビナントRabMAb

Anti-MAD1L1/MAD1 抗体 [EPR14676-23] (ab184560)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1L1/MAD1 antibody [EPR14676-23] (ab184560)
  • Western blot - Anti-MAD1L1/MAD1 antibody [EPR14676-23] (ab184560)
  • Anti-MAD1L1/MAD1 antibody [EPR14676-23] (ab184560)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14676-23] to MAD1L1/MAD1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MAD1L1/MAD1 antibody [EPR14676-23]
    MAD1L1/MAD1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14676-23] to MAD1L1/MAD1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2, A431, HeLa and MCF7 lysates. Human melanoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184560の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 83 kDa.
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 83 kDa.
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.

  • 組織特異性

    Expressed weakly at G0/G1 and highly at late S and G2/M phase.

  • 関連疾患

    Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.

  • 配列類似性

    Belongs to the MAD1 family.

  • 翻訳後修飾

    Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.

  • 細胞内局在

    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
  • Information by UniProt

  • 参照データベース

    • 別名

      • hMAD1 antibody
      • HsMAD1 antibody
      • MAD1 antibody
      • MAD1 mitotic arrest deficient like 1 (yeast) antibody
      • MAD1-like protein 1 antibody
      • MAD1L1 antibody
      • MD1L1_HUMAN antibody
      • Mitotic arrest deficient 1 antibody
      • Mitotic arrest deficient 1-like protein 1 antibody
      • Mitotic checkpoint MAD1 protein homolog antibody
      • Mitotic spindle assembly checkpoint protein MAD1 antibody
      • PIG9 antibody
      • Tax binding protein 181 antibody
      • Tax-binding protein 181 antibody
      • TP53I9 antibody
      • Tumor protein p53 inducible protein 9 antibody
      • TXBP181 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1L1/MAD1 antibody [EPR14676-23] (ab184560)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1L1/MAD1 antibody [EPR14676-23] (ab184560)

      Immunohistochemical analysis of formalin fixed and paraffin embedded Human melanoma tissue labeling MAD1  with ab184560 at 1/50 dilution, counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-MAD1L1/MAD1 antibody [EPR14676-23] (ab184560)
      Western blot - Anti-MAD1L1/MAD1 antibody [EPR14676-23] (ab184560)
      All lanes : Anti-MAD1L1/MAD1 antibody [EPR14676-23] (ab184560) at 1/20000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : A431 lysate
      Lane 3 : HeLa lysate
      Lane 4 : MCF7 lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 83 kDa

    • Anti-MAD1L1/MAD1 antibody [EPR14676-23] (ab184560)
      Anti-MAD1L1/MAD1 antibody [EPR14676-23] (ab184560)

    参考文献 (0)

    ab184560 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184560 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MAD1 antibody [EPR14676-23]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    No
    Specification
    HeLa
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    投稿 May 23 2017

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