Anti-MAD1L1/MAD1 抗体 (ab11691)
Key features and details
- Rabbit polyclonal to MAD1L1/MAD1
- Suitable for: WB, ICC/IF
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
製品の概要
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製品名
Anti-MAD1L1/MAD1 antibody
MAD1L1/MAD1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MAD1L1/MAD1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Saccharomyces cerevisiae -
免疫原
Recombinant full length protein (GST-tag) corresponding to Saccharomyces cerevisiae MAD1L1/MAD1 aa 1-750.
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ポジティブ・コントロール
- S.cerevisiae whole cell lysate (see images).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11691の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/100. Detects a band of approximately 83 kDa.
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|
ICC/IF |
Use at an assay dependent dilution.
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特記事項 |
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WB
1/100. Detects a band of approximately 83 kDa. |
ICC/IF
Use at an assay dependent dilution. |
ターゲット情報
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機能
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding. -
組織特異性
Expressed weakly at G0/G1 and highly at late S and G2/M phase. -
関連疾患
Defects in MAD1L1 are involved in the development and/or progression of various types of cancer. -
配列類似性
Belongs to the MAD1 family. -
翻訳後修飾
Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore. - Information by UniProt
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別名
- hMAD1 antibody
- HsMAD1 antibody
- MAD1 antibody
see all
画像
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All lanes : Anti-MAD1L1/MAD1 antibody (ab11691) at 1/100 dilution
Lane 1 : Wild type S. cerevisiae whole cell lysate
Lane 2 : mad1 knockout S. cerevisiae whole cell lysate
Developed using the ECL technique.
Observed band size: 83 kDa why is the actual band size different from the predicted? -
ab11691 at a 1/100 dilution staining MAD1 in S.cerevisiae by immunofluorescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab11691 は論文での使用が確認できていません。