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Anti-M13 Bacteriophage Coat Protein g8p 抗体 [RL-ph2] (ab9225)

Reviews (2)Q&A (6)References (4)
Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)

    Key features and details

    • Mouse monoclonal [RL-ph2] to M13 Bacteriophage Coat Protein g8p
    • Suitable for: ICC, Flow Cyt, WB
    • Isotype: IgG2a

    製品の概要

    • 製品名

      Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]

    • 製品の詳細

      Mouse monoclonal [RL-ph2] to M13 Bacteriophage Coat Protein g8p

    • 由来種

      Mouse

    • 特異性

      RL-ph2 reacts with the major M13 filamentous phage coat protein g8p with a molecular weight of 5 kDa.

    • アプリケーション

      適用あり: ICC, Flow Cyt, WBmore details

    • 種交差性

      交差種: Enterobacteria phage M13

    • 免疫原

      Recombinant full length protein corresponding to Enterobacteria phage M13 M13 Bacteriophage Coat Protein g8p. Isolated M13 phage coat proteins
      Database link: P69541

    • 特記事項


      The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • 精製度

      Protein A purified

    • 一次抗体 備考

      The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

    • ポリ/モノ

      モノクローナル

    • クローン名

      RL-ph2

    • ミエローマ

      Sp2/0-Ag14

    • アイソタイプ

      IgG2a

    • 軽鎖の種類

      kappa

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab9225の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ICC
    Use at an assay dependent concentration.
    Flow Cyt
    1/25 - 1/200.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
    WB (1)
    1/100 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).
    特記事項
    ICC
    Use at an assay dependent concentration.
    Flow Cyt
    1/25 - 1/200.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
    WB
    1/100 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).

    ターゲット情報

    • 関連性

      M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

    • 別名

      • Capsid protein G8P antibody
      • Coat protein B antibody
      • G8P antibody
      • Major coat protein antibody
      • VIII antibody
      see all

    画像

    • Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)
      Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)Image courtesy of an anonymous Abreview.
      Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) at 1/1000 dilution + whole cell lysate prepared from XL10Gold cells at 50 µg

      Secondary
      Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution

      Predicted band size: 5 kDa
      Observed band size: 6 kDa why is the actual band size different from the predicted?



      Primary antibody incubated for 16 hours at 4°C.
      Blocking step performed using 5% milk for 1 hour at room temperature.

      See Abreview

    参考文献 (4)

    ab9225 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9225 は 4 報の論文で使用されています。

    • Kellmann SJ  et al. SpyDisplay: A versatile phage display selection system using SpyTag/SpyCatcher technology. MAbs 15:2177978 (2023). PubMed: 36803166
    • Hulin-Curtis SL  et al. Identification of folate receptor a (FRa) binding oligopeptides and their evaluation for targeted virotherapy applications. Cancer Gene Ther 27:785-798 (2020). PubMed: 31902944
    • Meulemans EV  et al. Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody. Hum Antibodies Hybridomas 6:113-8 (1995). PubMed: 8597626
    • Meulemans EV  et al. Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody. J Mol Biol 244:353-60 (1994). PubMed: 7527464

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-8 of 8 Abreviews or Q&A

    Western blot abreview for Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Escherichia coli Cell lysate - whole cell (XL10Gold)
    Loading amount
    50 µg
    Specification
    XL10Gold
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 20 2010

    ELISA abreview for Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]

     Excellent
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Escherichia coli Cell (XL10Gold)
    Specification
    XL10Gold
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Type
    Direct
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 07 2009

    Question

    ear Sir Good day Do you offer the protein of M13 Bacteriophage Coat Protein g8p. The antigen for the product, https://www.abcam.com/M13-Bacteriophage-Coat-Protein-g8p-antibody-RL-ph2-ab9225.html Thank you. Best Regards

    Read More
    Answer

    Thank you for your enquiry and your interest. Currently, we only have antibodies against M13 Bacteriophage Coat Protein g8p but not the purified protein. If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Question

    ab9225 arrived warm and gives no signal in WB

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***.
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

    Question

    Phoned because the last lot did not work as effectively in a lateral flow assay.

    Read More
    Answer

    Thank you for contacting Abcam.

    I am sorry to hear of the issues that you have been experimenting with this latest lot of ab9225. I have had the opportunity to speak with my collegues about adding the lateral flow application to our Abreview program. Unfortunately this will not be possible at this time.

    As I mentioned on the phone, this product has not been tested in the type of assay that you have been performing and as such we cannot guarantee that it will give satifactor results from lot to lot. I am sorry for any difficulties that this has caused.

    Please let me know if there if anything else that we may do to help you meet your research goals.

    Read More

    Question

    Dear Sir  Good day Do you offer the protein of M13 Bacteriophage Coat Protein g8p. The antigen for the product, https://www.abcam.com/M13-Bacteriophage-Coat-Protein-g8p-antibody-RL-ph2-ab9225.html  Thank you.  Best Regards

    Read More
    Answer

    Thank you for contacting us. Unfortunately we currently do not have any M13 Bacteriophage Coat g8p protein in our catalogue. I'm sorry I could not have been of more help but if you have any further questions please do not hesitate to ask.

    Read More

    Question

    Do you have antibodies for phage proteins?

    Read More
    Answer

    Thank you for your reply. We have a number of antibodies to Bacteriophage Proteins such as ab6188: Anti-M13 + Fd Bacteriophage Coat Proteins antibody or ab9225: Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]. I hope this helps, please let me know if you need any additional information.

    Read More

    Question

    I was wondering if your mouse monoclonal M13 bacteriophage coat protein g8p antibodies (ab9225, ab9224 or ab9223) come in smaller sizes than the 1ml aliqots? I have been using the equivelent Serotech antibody for various applications including WB, immunoprecipitation and immunocytoshemistry, but would like to try your the AbCam version as I have been having problems, but 1ml is cost prohibitive. Suzanne.

    Read More
    Answer

    Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

    Read More

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