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AB9225

Anti-M13 Bacteriophage Coat Protein g8p 抗体 [RL-ph2]

Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]

4

(3 Reviews)

|

(4 Publications)

Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) is a mouse monoclonal antibody detecting M13 Bacteriophage Coat Protein g8p in Western Blot, Flow Cytometry. Suitable for Enterobacteria phage M13.

- Trusted since 2002

別名を表示する

Capsid protein G8P, Coat protein B, Gene 8 protein, M13 procoat, Major coat protein, G8P, VIII

1 Images
Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (AB9225)
  • WB

AbReview20684****

Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (AB9225)

Primary antibody incubated for 16 hours at 4°C.
Blocking step performed using 5% milk for 1 hour at room temperature.

All lanes:

Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) at 1/1000 dilution

All lanes:

whole cell lysate prepared from XL10Gold cells at 50 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (<a href='/products/secondary-antibodies/rabbit-mouse-igg-h-l-hrp-ab6728'>ab6728</a>) at 1/2000 dilution

Predicted band size: 8 kDa

Observed band size: 6 kDa

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Image courtesy of an anonymous Abreview.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

RL-ph2

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Enterobacteria phage M13

アプリケーション

Flow Cyt, WB, ICC

applications

免疫原

Recombinant Full Length Protein corresponding to Enterobacteria phage M13 Capsid protein G8P.

P69541

特異性

RL-ph2 reacts with the major M13 filamentous phage coat protein g8p with a molecular weight of 5 kDa.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Enterobacteria phage M13": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/25 - 1/200", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>" }, "Recombinant full length protein - Enterobacteria phage M13": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }
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製品の詳細

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
バッファー組成
Preservative: 0.09% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The M13 bacteriophage coat protein g8p also known simply as g8p plays a mechanical role in forming the protective sheath around the phage's single-stranded DNA. The molecular weight of g8p is approximately 5 kDa. It is an important component of the M13 bacteriophage typically expressed within the bacterial host after phage infection. As one of the coat proteins g8p drives the assembly of new phage particles by arranging itself into helical structures that encapsulate the viral genome.
Biological function summary

G8p's function extends beyond just structural support for the phage. It participates in the assembly of the M13 phage which is part of the extrinsic complex necessary for phage propagation. During this process g8p cooperates closely with other proteins to manage the packaging of genetic material and ensure successful phage extrusion from the bacterial host. Functionally g8p aids in maintaining phage integrity and viability directly affecting the efficiency of phage propagation within the microbial environment.

Pathways

G8p has an essential role in the M13 bacteriophage lifecycle. It facilitates the phage's extrusion pathway where newly formed phage particles exit the bacterial cell. This pathway involves interactions with proteins like the M13 p9 (g9p) protein ensuring efficient phage release and infection of nearby bacterial cells. The interaction among these proteins through the extrusion pathway is critical for the replication and spread of the M13 bacteriophage.

G8p as a component of phage display technology interacts indirectly with a variety of disorders particularly bacterial infections. M13 phage display systems use g8p to present peptides or proteins on the phage surface aiding in identifying molecules that can target infectious agents. Although g8p's primary function does not link directly to specific diseases its application in phage display connects it with therapeutic and diagnostic developments potentially interacting with proteins involved in bacterial pathogenesis.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane.
See full target information Capsid protein G8P
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

mAbs 15:2177978 PubMed36803166

2023

SpyDisplay: A versatile phage display selection system using SpyTag/SpyCatcher technology.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah-Jane Kellmann,Christian Hentrich,Mateusz Putyrski,Hanh Hanuschka,Manuel Cavada,Achim Knappik,Francisco Ylera

Cancer gene therapy : PubMed31902944

2020

Identification of folate receptor α (FRα) binding oligopeptides and their evaluation for targeted virotherapy applications.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah L Hulin-Curtis,James A Davies,Davor Nestić,Emily A Bates,Alexander T Baker,Tabitha G Cunliffe,Dragomira Majhen,John D Chester,Alan L Parker

Human antibodies and hybridomas 6:113-8 PubMed8597626

1995

Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody.

Applications

Unspecified application

Species

Unspecified reactive species

E V Meulemans,L J Nieland,W H Debie,F C Ramaekers,G J van Eys

Journal of molecular biology 244:353-60 PubMed7527464

1994

Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody.

Applications

Unspecified application

Species

Unspecified reactive species

E V Meulemans,R Slobbe,P Wasterval,F C Ramaekers,G J van Eys
View all publications
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