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リコンビナントRabMAb

Anti-M-CSF 抗体 [EP1179Y] (ab52864)

Submit a review Q&A (1)References (25)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)
  • Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] (ab52864)
  • Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
  • Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EP1179Y] (ab52864)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)
  • Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
  • Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (ab52864)
  • Anti-M-CSF antibody [EP1179Y] (ab52864)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1179Y] to M-CSF
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Reacts with: Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP

製品の概要

  • 製品名

    Anti-M-CSF antibody [EP1179Y]
    M-CSF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1179Y] to M-CSF

  • 由来種

    Rabbit

  • 特異性

    This antibody fails to detect endogenous natural samples in WB.

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human, Recombinant fragment
    交差が予測される動物種: Sheep, Cow, Chimpanzee

  • 免疫原

    Synthetic peptide within Human M-CSF aa 50-150. The exact sequence is proprietary.
    Database link: P09603

  • ポジティブ･コントロール

    • WB: Recombinant MCSF IHC-P: Human tonsil tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab52864の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/80.
WB
1/2000. Predicted molecular weight: 60 kDa.

This antibody fails to detect endogenous natural samples in WB.
IP
1/40.

For unpurified use at 1/50 dilution. 
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100 dilution.
ICC/IF
1/100.

For unpurified use at 1/250 - 1/500 dilution.
特記事項
Flow Cyt (Intra)
1/80.
WB
1/2000. Predicted molecular weight: 60 kDa.

This antibody fails to detect endogenous natural samples in WB.
IP
1/40.

For unpurified use at 1/50 dilution. 
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100 dilution.
ICC/IF
1/100.

For unpurified use at 1/250 - 1/500 dilution.

ターゲット情報

  • 機能

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.

  • 翻訳後修飾

    Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.

  • 細胞内局在

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Colony stimulating factor 1 (macrophage) antibody
      • Colony stimulating factor 1 antibody
      • Colony stimulating factor macrophage specific antibody
      • CSF 1 antibody
      • CSF-1 antibody
      • CSF1 antibody
      • CSF1_HUMAN antibody
      • Csfm antibody
      • Lanimostim antibody
      • M CSF antibody
      • M-CSF antibody
      • Macrophage Colony Stimulating Factor 1 antibody
      • Macrophage colony stimulating factor antibody
      • MCSF antibody
      • MGC31930 antibody
      • Processed macrophage colony-stimulating factor 1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling M-CSF with Purified ab52864 at 1:500 dilution (1.52 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    • Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] (ab52864)

      Immunocytochemistry/ Immunofluorescence analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with Purified ab52864 at 1:100 (7.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Anti-M-CSF antibody [EP1179Y] (ab52864) at 1/2000 dilution (Purified) + Human M-CSF (aa 33-190) recombinant protein at 0.015 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 60 kDa
      Observed band size: 19 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EP1179Y] (ab52864)

      Intracellular Flow Cytometry analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with purified ab52864 at 1/80 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr®488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for M-CSF with unpurified ab52864  (1/50 dilution) in immunohistochemical analysis.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Anti-M-CSF antibody [EP1179Y] (ab52864) at 1/50000 dilution (unpurified) + recombinant MCSF at 0.005 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 60 kDa
      Observed band size: 20 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (ab52864)

      ab52864 (purified) at 1:40 dilution (2µg) immunoprecipitating M-CSF in THP-1 whole cell lysate.
      Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10µg
      Lane 2 (+): ab52864 & THP-1 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52864 in THP-1 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Anti-M-CSF antibody [EP1179Y] (ab52864)
      Anti-M-CSF antibody [EP1179Y] (ab52864)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (25)

    ab52864 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab52864 は 25 報の論文で使用されています。

    • Honda H  et al. Genome-Wide Gene Expression Profiling Reveals the Direct Effect of Dienogest on Ovarian Endometriotic Stromal Cells. Reprod Sci 30:2457-2467 (2023). PubMed: 36752987
    • Chandler AC  et al. Neoplastic synovial lining cells that coexpress podoplanin and CD90 overproduce CSF-1, driving tenosynovial giant cell tumor. J Orthop Res 40:1918-1925 (2022). PubMed: 34855235
    • Nakajima S  et al. Neoadjuvant Chemotherapy Induces IL34 Signaling and Promotes Chemoresistance via Tumor-Associated Macrophage Polarization in Esophageal Squamous Cell Carcinoma. Mol Cancer Res 19:1085-1095 (2021). PubMed: 33674443
    • Back J  et al. Inflammatory conversion of quiescent osteoblasts by metastatic breast cancer cells through pERK1/2 aggravates cancer-induced bone destruction. Bone Res 9:43 (2021). PubMed: 34588427
    • Steins A  et al. High-grade mesenchymal pancreatic ductal adenocarcinoma drives stromal deactivation through CSF-1. EMBO Rep 21:e48780 (2020). PubMed: 32173982
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    I've been using your antibody against M-CSF (ab52864). It says it is a 60kDa protein but is observed at 20kDa. When tested in my samples, this protein is coming up at around 45kDa. Have you seen this before? It is a very clean blot with only one specific band present so I do believe the antibody is very specific but just wondering if you have any advice in this case?


     



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    Answer

    I can confirm to you that it is reported in literature that M-CSF can also be detected in a 45 kDa form (see links below)

    https://www.ncbi.nlm.nih.gov/pubmed/2179407

    https://www.researchgate.net/publication/14333823_Macrophage_colony_stimulating_factor_involvement_in_uremic_patients  (in the introduction section).

    The size of the band is dependent on whether you are detecting the circulating or membrane bound form.

    I hope you found this information helpful and I am happy to answer any further questions or concerns you have.  The antibody is covered by the Abpromise guarantee.

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