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リコンビナントRabMAb

Anti-M Cadherin 抗体 [EPR25401-63] - BSA and Azide free (ab290747)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)
  • Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25401-63] to M Cadherin - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free
    M Cadherin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25401-63] to M Cadherin - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: ICC/IF,IHC-Fr,IP or WB

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: satellite cells of mouse skeletal muscle, granule cells of mouse cerebellum, rat skeletal muscle tissue.

  • 特記事項

    ab290747 is a carrier free version of ab290732. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290747の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.

1) Tissues samples were incubated with 3% hydrogen peroxide after secondary antibody incubation to reduce the background.

2) Non-specific staining was observed in 3 cases (rat liver, rat colon and mouse colon) from a mouse and rat TMA containing 36 tissue samples.
特記事項
IHC-P
Use at an assay dependent concentration.

1) Tissues samples were incubated with 3% hydrogen peroxide after secondary antibody incubation to reduce the background.

2) Non-specific staining was observed in 3 cases (rat liver, rat colon and mouse colon) from a mouse and rat TMA containing 36 tissue samples.
追加情報
Is unsuitable for ICC/IF,IHC-Fr,IP or WB.

ターゲット情報

  • 機能

    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation.

  • 組織特異性

    Expressed in the brain and cerebellum.

  • 関連疾患

    A chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24).
    Mental retardation, autosomal dominant 3

  • 配列類似性

    Contains 5 cadherin domains.

  • ドメイン

    Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CAD15_HUMAN antibody
      • Cadherin 14 antibody
      • Cadherin 15 antibody
      • Cadherin 15, type 1, M cadherin (myotubule) antibody
      • Cadherin 3 antibody
      • Cadherin-14 antibody
      • Cadherin-15 antibody
      • Cadherin14 antibody
      • Cadherin15 antibody
      • Cadherin3 antibody
      • CCAD antibody
      • CDH 14 antibody
      • CDH 15 antibody
      • CDH 3 antibody
      • CDH14 antibody
      • Cdh15 antibody
      • CDH3 antibody
      • CDHM antibody
      • M-cadherin antibody
      • MCAD antibody
      • MRD3 antibody
      • Muscle cadherin antibody
      • Myotubule cadherin antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)

      This data was developed using ab290732, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labelling M Cadherin with ab290732 at 1/4000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) as a secondary antibody. Positive staining on satellite cells of mouse skeletal muscle. The section was incubated with ab290732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.  Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

      Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)

      This data was developed using ab290732, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling M Cadherin with ab290732 at 1/4000 (0.114 ug/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on granule cells of mouse cerebellum. The section was incubated with ab290732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

      Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)

      This data was developed using ab290732, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labelling M Cadherin with ab290732 at 1/4000 dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on satellite cells of rat skeletal muscle. The section was incubated with ab290732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

      Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)

      This data was developed using ab290732, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling M Cadherin with ab290732 at 1/4000 (0.114 ug/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Negative control: no staining on mouse cerebrum. The section was incubated with ab290732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

      Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

    • Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)
      Anti-M Cadherin antibody [EPR25401-63] - BSA and Azide free (ab290747)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab290747 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290747 は論文での使用が確認できていません。

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