Anti-M Cadherin 抗体 [EPR25401-63] (ab290732)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] (ab290732)](https://www.abcam.co.jp/ps/products/290/ab290732/Images/ab290732-2-anti-m-cadherin-antibody-epr2540163-immunohistochemistry-mouse-skeletal-muscl.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] (ab290732)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25401-63] to M Cadherin
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-M Cadherin antibody [EPR25401-63]
M Cadherin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25401-63] to M Cadherin -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details
適用なし: ICC/IF,IHC-Fr,IP or WB -
種交差性
交差種: Mouse, Rat
非交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-P: satellite cells of mouse skeletal muscle, granule cells of mouse cerebellum, rat skeletal muscle tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25401-63 -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab290732の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/4000.
1) Tissues samples were incubated with 3% hydrogen peroxide after secondary antibody incubation to reduce the background. 2) Non-specific staining was observed in 3 cases (rat liver, rat colon and mouse colon) from a mouse and rat TMA containing 36 tissue samples. |
| 特記事項 |
|---|
|
IHC-P
1/4000. 1) Tissues samples were incubated with 3% hydrogen peroxide after secondary antibody incubation to reduce the background. 2) Non-specific staining was observed in 3 cases (rat liver, rat colon and mouse colon) from a mouse and rat TMA containing 36 tissue samples. |
ターゲット情報
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機能
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation. -
組織特異性
Expressed in the brain and cerebellum. -
関連疾患
A chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24).
Mental retardation, autosomal dominant 3 -
配列類似性
Contains 5 cadherin domains. -
ドメイン
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 12555 Mouse
- Entrez Gene: 361432 Rat
- SwissProt: P33146 Mouse
- Unigene: 1976 Mouse
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別名
- CAD15_HUMAN antibody
- Cadherin 14 antibody
- Cadherin 15 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] (ab290732)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labelling M Cadherin with ab290732 at 1/4000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) as a secondary antibody. Positive staining on satellite cells of mouse skeletal muscle. The section was incubated with ab290732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] (ab290732)](https://www.abcam.co.jp/ps/products/290/ab290732/Images/ab290732-3-anti-m-cadherin-antibody-epr2540163-immunohistochemistry-mouse-cerebellum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] (ab290732)Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling M Cadherin with ab290732 at 1/4000 (0.114 ug/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on granule cells of mouse cerebellum. The section was incubated with ab290732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] (ab290732)](https://www.abcam.co.jp/ps/products/290/ab290732/Images/ab290732-4-anti-m-cadherin-antibody-epr2540163-immunohistochemistry-rat-skeletal-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] (ab290732)Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labelling M Cadherin with ab290732 at 1/4000 dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on satellite cells of rat skeletal muscle. The section was incubated with ab290732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] (ab290732)](https://www.abcam.co.jp/ps/products/290/ab290732/Images/ab290732-5-anti-m-cadherin-antibody-epr2540163-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M Cadherin antibody [EPR25401-63] (ab290732)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling M Cadherin with ab290732 at 1/4000 (0.114 ug/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Negative control: no staining on mouse cerebrum. The section was incubated with ab290732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
-
![Anti-M Cadherin antibody [EPR25401-63] (ab290732)](https://www.abcam.co.jp/ps/products/290/ab290732/Images/ab290732-484500-Benefits-of-Recombinant-antibodies.jpg)
Anti-M Cadherin antibody [EPR25401-63] (ab290732)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab290732 は論文での使用が確認できていません。