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リコンビナントRabMAb

Anti-LZTR1 抗体 [EPR25253-51] - BSA and Azide free (ab289988)

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Flow Cytometry (Intracellular) - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
  • Immunocytochemistry/ Immunofluorescence - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
  • Immunoprecipitation - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
  • Western blot - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
  • Immunoprecipitation - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
  • Western blot - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
  • Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25253-51] to LZTR1 - BSA and Azide free
  • Suitable for: IP, ICC/IF, Flow Cyt (Intra), WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free
    LZTR1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25253-51] to LZTR1 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, ICC/IF, Flow Cyt (Intra), WBmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, K562, SH-sY5Y and RAW264.7 whole cell lysates. ICC/IF: SH-SY5Y cells. Flow cyt: SH-SY5Y cells. IP: HEK-293T and NIH/3T3 whole cell lysates.

  • 特記事項

    ab289988 is the carrier-free version of ab289965

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289988の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).
特記事項
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Probable transcriptional regulator that may play a crucial role in embryogenesis.

  • 配列類似性

    Contains 2 BTB (POZ) domains.
    Contains 6 Kelch repeats.

  • 発生段階

    Expressed in fetal brain, heart, kidney, liver and lung.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Leucine zipper like regulator 1 antibody
      • Leucine zipper like transcription regulator 1 antibody
      • Leucine-zipper-like transcriptional regulator 1 antibody
      • LZTR-1 antibody
      • Lztr1 antibody
      • LZTR1_HUMAN antibody
      • MGC21205 antibody
      • TCFL2 antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
      Flow Cytometry (Intracellular) - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)

      This data was developed using ab289965, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling LZTR1 with ab289965 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

    • Immunocytochemistry/ Immunofluorescence - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
      Immunocytochemistry/ Immunofluorescence - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)

      This data was developed using ab289965, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling LZTR1 with ab289965 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasm staining in SH-SY5Y cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

    • Immunoprecipitation - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
      Immunoprecipitation - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)

      This data was developed using ab289965, the same antibody clone in a different buffer formulation.

      LZTR1 was immunoprecipitated from NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab289965 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289965 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate10 µg

      Lane 2: ab289965 IP in NIH/3T3 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289965 in NIH/3T3 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 41 seconds

       

    • Western blot - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
      Western blot - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
      All lanes : Anti-LZTR1 antibody [EPR25253-51] (ab289965) at 1/1000 dilution

      Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
      Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
      Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100000 dilution

      Predicted band size: 94 kDa
      Observed band size: 95 kDa why is the actual band size different from the predicted?



      This data was developed using ab289965, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The bands nearby 50 kDa could be non-specific bands.

      Exposure times: Lane 1: 70 seconds; Lanes 2-3: 180 seconds.

    • Immunoprecipitation - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
      Immunoprecipitation - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)

      This data was developed using ab289965, the same antibody clone in a different buffer formulation.

      LZTR1 was immunoprecipitated from HEK-293T ( human embryonic kidney epithelial cell) whole cell lysate with ab289965 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289965 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: HEK-293T ( human embryonic kidney epithelial cell) whole cell lysate 10 µg

      Lane 2: ab289965 IP in HEK-293T whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289965 in HEK-293T whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 41 seconds

       

    • Western blot - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
      Western blot - Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
      All lanes : Anti-LZTR1 antibody [EPR25253-51] (ab289965) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
      Lane 2 : HeLa transfected with siRNA specifically targeting LZTR1 whole cell lysate

      Lysates/proteins at 12 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 94 kDa
      Observed band size: 95 kDa why is the actual band size different from the predicted?



      This data was developed using ab289965, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The bands nearby 50 kDa could be non-specific bands.

      Exposure time: 3 minutes.

    • Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)
      Anti-LZTR1 antibody [EPR25253-51] - BSA and Azide free (ab289988)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab289988 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab289988 は論文での使用が確認できていません。

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