Anti-LZTFL1 抗体 (ab187918)
Key features and details
- Rabbit polyclonal to LZTFL1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-LZTFL1 antibody
LZTFL1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LZTFL1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Cynomolgus monkey, Orangutan
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免疫原
Recombinant fragment corresponding to Human LZTFL1 aa 50-200.
Database link: Q9NQ48 -
ポジティブ･コントロール
- IHC-P: Human testis, skeletal muscle, lymph node and liver tissue; WB: RT4 and U-251 MG whole cell lysates; ICC: U-2 OS whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187918の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.
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| IHC-P |
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa. |
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IHC-P
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 512570 Cow
- Entrez Gene: 54585 Human
- Entrez Gene: 93730 Mouse
- Entrez Gene: 316102 Rat
- Omim: 606568 Human
- SwissProt: Q3ZBL4 Cow
- SwissProt: Q4R6V9 Cynomolgus monkey
- SwissProt: Q9NQ48 Human
see all -
別名
- 5530402H04Rik antibody
- 6130400H19Rik antibody
- AI414725 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LZTFL1 antibody (ab187918)
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling LZTFL1 (high expression) with ab187918 at 1/2500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-LZTFL1 antibody (ab187918)All lanes : Anti-LZTFL1 antibody (ab187918) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
Predicted band size: 35 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-LZTFL1 antibody (ab187918)Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling LZTFL1 in the cytosol with ab187918 at 1/2500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LZTFL1 antibody (ab187918)Immunohistochemical analysis of paraffin-embedded human lymph node tissue labeling LZTFL1 with ab187918 at 1/2500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LZTFL1 antibody (ab187918)Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling LZTFL1 (low expression) with ab187918 at 1/2500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LZTFL1 antibody (ab187918)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling LZTFL1 with ab187918 at 1/2500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab187918 は論文での使用が確認できていません。