Anti-LYVE1 抗体 (ab33682)
Key features and details
- Rabbit polyclonal to LYVE1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LYVE1 antibody
LYVE1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LYVE1 -
由来種
Rabbit -
特異性
From July 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: IHC-P, ICC/IFmore details
適用なし: WB -
種交差性
交差種: Mouse
交差が予測される動物種: Cow, Pig
非交差種:
Human -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Mouse LYVE1.
Immunogen の所有権に関して (Peptide available as ab34093.) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33682の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (10) |
Use a concentration of 5 µg/ml.
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 5 µg/ml. |
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ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. -
組織特異性
Mainly expressed in endothelial cells lining lymphatic vessels. -
配列類似性
Contains 1 Link domain. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface. - Information by UniProt
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参照データベース
- Entrez Gene: 404179 Cow
- Entrez Gene: 114332 Mouse
- Entrez Gene: 397626 Pig
- SwissProt: Q8BHC0 Mouse
- Unigene: 396078 Mouse
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別名
- Cell surface retention sequence-binding protein 1 antibody
- CRSBP 1 antibody
- CRSBP-1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab33682)
IHC image of LYVE1 staining in a section of formalin-fixed paraffin-embedded normal human colon* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab33682, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab33682)IHC image of LYVE1 staining in a section of formalin-fixed paraffin-embedded normal mouse large intestine performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab33682, 1 µg/mL, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (47)
ab33682 は 47 報の論文で使用されています。
- Peng J et al. Individual variation in and lateral asymmetry of mouse epididymal draining lymph nodes. Am J Reprod Immunol 89:e13678 (2023). PubMed: 36648083
- Huang B et al. ANRIL promotes the regulation of colorectal cancer on lymphatic endothelial cells via VEGF-C and is the key target for Pien Tze Huang to inhibit cancer metastasis. Cancer Gene Ther 30:1260-1273 (2023). PubMed: 37286729
- Dalaei F et al. Short term treatment of secondary lymphedema with hyaluronidase injections reduces mouse hindlimb lymphedema. J Plast Surg Hand Surg 58:40-47 (2023). PubMed: 37338078
- Daniel L et al. L-selectin-dependent and -independent homing of naïve lymphocytes through the lung draining lymph node support T cell response to pulmonary Mycobacterium tuberculosis infection. PLoS Pathog 19:e1011460 (2023). PubMed: 37405965
- Rosa I et al. Lymphatic Endothelial-to-Myofibroblast Transition: A Potential New Mechanism Underlying Skin Fibrosis in Systemic Sclerosis. Cells 12:N/A (2023). PubMed: 37681927