Anti-LYVE1 抗体 (ab10278)

Thank you for your interest in our LYVE1 antibody ab10278. We do not have any small samples but our guarantee applies to IHC on paraffin-embedded or frozen human tissue. We do not have any data for prostate tissue stained with ab10278 but LYVE1 has been used as a marker for lymphatic vessels in prostate tissue using other antibodies. Antibody ab10278 should be able to detect LYVE1 in prostate as well as it detects LYVE1 in the colon tissue we show on the datasheet, as long as you include a heat-mediated antigen retrieval step.

Thank you for your inquiry.

We did not have an MSDS beforehands, as the product does not contain any chemicals requiring an MSDS. However we have uploaded now the MSDS for you - you can find it now on the website. https://www.abcam.com/LYVE1-antibody-ab10278.html (or use the following: https://www.abcam.com/LYVE1-antibody-ab10278.html).

I hope this is helpful. Please do not hesitate to contact us again should you have any additional questions.

My apologies for this problem. Some people indeed have had problems with this antibody in IHC-P (this application came from a customer review) and we believe the antibody may be difficult to optimise, hence we have removed this application from the datasheet. We have had better results with the immunogen affinity purified version of the antibody (ab36993) which has been successfully tested in IHC-P. I have asked our accounting department to issue you a full refund. I hope that this experience will not prevent you from purchasing a product from us in the future as we have many quality products. If you have any further questions concerning your refund, please contact the accounting department at accounts@abcam.com. The technical team is always at your service, should you require further expert advice.

Thank you for your enquiry. I am sorry to hear that you have been experiencing difficulties with this antibody. I have read through your technical questionaire and I have a few comments. The application of immunohistochemistry was added to the list of applications associated with this antibody following an excellent review from one of our customers. I would like to recommend that you apply the basics of this protocol to your tissues. They employed a 16 hours fixation with a deparaffination in Neoclear, a rehydration in a dilution series followed by a peroxidase block and antigen retrieval in citrate retrieval using the microwave method. I have pasted the protocol that was used below. Of particular attention the reviewer mentioed that "the main concern of this technique are the re-hydration steps; the tissue(s) must never be left to dry, and the re-hydration should be done stepwise (at least 4 ethanol dilutions, 5-10 mins each). For thyroid tumors, blocking with hydrogenous peroxide is also crucial." The protocol is as follows: Use Paraffin enbeded samples - section thickness 3?m - deparaffinate 3 X 5 min in Neoclear (or equivalent) - rehydrate with alcohol (100% -96% -80% -70%, 5 minutes in every solution ) then place in water - block peroxidase in 1% H2O2 /methanol for 5 min. - wash 2 x in PBS, 2x5 min in citrate-buffer / microwave 600 watt - cool down in citrate-buffer for 15-20 min. - wash 1 x in PBS - Wash 2 x with PBS / 0.1%Tween - Incubate with primary antibody (1:400 dillution) overnight (18-20 hours) at 4oC in a humidified chamber - wash 2 x in PBS/0.1%Tween - goat anti rabbit Ab (any source, Santa Cruz for instance) for 60 min. in humid chamber at room temperature - wash 2 x with PBS/0.1%Tween - reveal secondary staining with secondary reagents (any source) - wash under streaming Distilled water (5 min) - counter stain and mount slides If you continue to have difficulty, please get back in touch with me.

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with rabbit has not yet been tested for use with ab10278. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 points will be offered for an image). Please let me know if you require any further assistance.