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リコンビナントRabMAb

Anti-LYPD3 抗体 [EPR9107] (ab151709)

Submit a review Q&A (1)References (4)
Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (ab151709)
  • Immunoprecipitation - Anti-LYPD3 antibody [EPR9107] (ab151709)
  • Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (ab151709)
  • Anti-LYPD3 antibody [EPR9107] (ab151709)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9107] to LYPD3
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-LYPD3 antibody [EPR9107]
    LYPD3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR9107] to LYPD3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IPmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human LYPD3. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • BxPC3, HACAT cell lysates, Human skin tissue, Human esophagus lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR9107

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab151709の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 36 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.
IP
1/10 - 1/100.
特記事項
WB
1/1000. Predicted molecular weight: 36 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.
IP
1/10 - 1/100.

ターゲット情報

  • 機能

    Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression.

  • 組織特異性

    Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression is found in the tumor component of some noninvasive superficial lesions and in invasive and metastatic urothelial cancers.

  • 配列類似性

    Contains 2 UPAR/Ly6 domains.

  • 翻訳後修飾

    N-glycosylated and O-glycosylated.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 2310061G07Rik antibody
      • C4.4A antibody
      • GPI-anchored metastasis-associated protein C4.4A homolog antibody
      • GPI-anchored metastasis-associated protein homolog antibody
      • Ly6/PLAUR domain-containing protein 3 antibody
      • Lypd3 antibody
      • LYPD3_HUMAN antibody
      • Matrigel-induced gene C4 protein antibody
      • MIG-C4 antibody
      • SHINC3 antibody
      see all

    画像

    • Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709)
      Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709)
      All lanes : Anti-LYPD3 antibody [EPR9107] (ab151709) at 1/1000 dilution (purified)

      Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysate
      Lane 2 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate
      Lane 3 : Human esophagus tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/5000 dilution

      Predicted band size: 36 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (ab151709)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (ab151709)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue sections labeling LYPD3 with Purified ab151709 at 1:1000 dilution (1.01 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Immunoprecipitation - Anti-LYPD3 antibody [EPR9107] (ab151709)
      Immunoprecipitation - Anti-LYPD3 antibody [EPR9107] (ab151709)

      ab151709 (purified) at 1:50 dilution (2ug) immunoprecipitating LYPD3 in HaCaT (Human skin keratinocyte) whole cell lysate.
      Lane 1 (input): HaCaT (Human skin keratinocyte) whole cell lysate 10ug
      Lane 2 (+): ab151709 & HaCaT (Human skin keratinocyte) whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151709 in HaCaT (Human skin keratinocyte) whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709)
      Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709)
      All lanes : Anti-LYPD3 antibody [EPR9107] (ab151709) at 1/1000 dilution (unpurified)

      Lane 1 : BxPC3 cell lysate
      Lane 2 : HACAT cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 36 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (ab151709)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (ab151709)

      Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling LYPD3 with unpurified ab151709 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-LYPD3 antibody [EPR9107] (ab151709)
      Anti-LYPD3 antibody [EPR9107] (ab151709)

    参考文献 (4)

    ab151709 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab151709 は 4 報の論文で使用されています。

    • Lin W  et al. Identification and validation of cellular senescence patterns to predict clinical outcomes and immunotherapeutic responses in lung adenocarcinoma. Cancer Cell Int 21:652 (2021). PubMed: 34872577
    • Gruet M  et al. ß2-Adrenergic Signalling Promotes Cell Migration by Upregulating Expression of the Metastasis-Associated Molecule LYPD3. Biology (Basel) 9:N/A (2020). PubMed: 32098331
    • Cocce KJ  et al. The Lineage Determining Factor GRHL2 Collaborates with FOXA1 to Establish a Targetable Pathway in Endocrine Therapy-Resistant Breast Cancer. Cell Rep 29:889-903.e10 (2019). PubMed: 31644911
    • Chen L  et al. TRAF6 regulates tumour metastasis through EMT and CSC phenotypes in head and neck squamous cell carcinoma. J Cell Mol Med 22:1337-1349 (2018). PubMed: 29193723

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    Customer kindly contacted is to inquire about the epitope region for these products.

    Read More
    Answer

    I have been informed by the lab that the immunogen used for creation of ab151709 is not N terminal but is instead located internally nearer the C terminus. The second rabbit monoclonal Ab155080 has been created from an immunogen corresponding to an area near the N terminus.

    Read More

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