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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Lyn 抗体 [Y497] (ab32398)

Submit a review Q&A (1)References (3)
Western blot - Anti-Lyn antibody [Y497] (ab32398)
  • Western blot - Anti-Lyn antibody [Y497] (ab32398)
  • Western blot - Anti-Lyn antibody [Y497] (ab32398)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y497] to Lyn
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Lyn antibody [Y497]
    Lyn 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [Y497] to Lyn

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Lyn aa 1-100. The exact sequence is proprietary.
    Database link: P07948

  • エピトープ

    Residues close to SH3 domain of human Lyn

  • ポジティブ･コントロール

    • WB: HAP1, Ramos, PC-12, and A431 whole cell lysates. Mouse lung, and mouse and rat liver tissue lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    Y497

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32398の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 53 kDa).

For unpurified use at 1/2000.
特記事項
WB
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 53 kDa).

For unpurified use at 1/2000.

ターゲット情報

  • 機能

    Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents.

  • 組織特異性

    Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.

  • ドメイン

    The protein kinase domain plays an important role in its localization in the cell membrane.

  • 翻訳後修飾

    Ubiquitinated. Ubiquitination is SH3-dependent.

  • 細胞内局在

    Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport.
  • Information by UniProt

  • 参照データベース

    see all

  • 製品の状態

    This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.

  • 別名

    • AA407514 antibody
    • EC 2.7.10.2 antibody
    • FLJ26625 antibody
    • Hck 2 antibody
    • JTK 8 antibody
    • JTK8 antibody
    • Lck/Yes related novel protein tyrosine kinase antibody
    • LYN antibody
    • LYN proto oncogene, Src family tyrosine kinase antibody
    • LYN_HUMAN antibody
    • ONCOGENE LYN antibody
    • p53Lyn antibody
    • p56Lyn antibody
    • Tyrosine protein kinase LYN antibody
    • Tyrosine-protein kinase Lyn antibody
    • V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody
    • Yamaguchi sarcoma viral (v yes 1) related oncogene homolog antibody
    see all

画像

  • Western blot - Anti-Lyn antibody [Y497] (ab32398)
    Western blot - Anti-Lyn antibody [Y497] (ab32398)
    All lanes : Anti-Lyn antibody [Y497] (ab32398) at 1/1000 dilution (Purified)

    Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
    Lane 2 : Mouse lung lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 53 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

  • Western blot - Anti-Lyn antibody [Y497] (ab32398)
    Western blot - Anti-Lyn antibody [Y497] (ab32398)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Lyn knockout HAP1 cell lysate (20 µg)
    Lane 3: Ramos cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab32398 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32398 was shown to specifically react with Lyn when Lyn knockout samples were used. Wild-type and Lyn knockout samples were subjected to SDS-PAGE. ab32398 and ab8245 (loading control to GAPDH) were diluted at 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Lyn antibody [Y497] (ab32398)
    Western blot - Anti-Lyn antibody [Y497] (ab32398)
    All lanes : Anti-Lyn antibody [Y497] (ab32398) at 1/2000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab32398 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa. 

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab32398 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (3)

ab32398 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab32398 は 3 報の論文で使用されています。

  • Wang X  et al. SNX10-mediated LPS sensing causes intestinal barrier dysfunction via a caspase-5-dependent signaling cascade. EMBO J 40:e108080 (2021). PubMed: 34747049
  • Liu Q  et al. Proteomic profiling for plasma biomarkers of tuberculosis progression. Mol Med Rep 18:1551-1559 (2018). PubMed: 29901122
  • Lee EJ  et al. Capsiate inhibits ultraviolet B-induced skin inflammation by inhibiting Src family kinases and epidermal growth factor receptor signaling. Free Radic Biol Med 48:1133-43 (2010). PubMed: 20123015

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

Question

We are currently using an antibody (phospho-Lyn (Tyr507)) that detects the inactivated form of Lyn. Which form of Lyn does this antibody dectect?

Read More
Answer

Thank you for your enquiry. I can confirm that ab32398 Lyn antibody should detect both versions of Lyn. I hope this information willbe useful for you.

Read More

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