AB182138

Anti-LYAR 抗体 [EPR14352-9]

Anti-LYAR antibody [EPR14352-9]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal LYAR antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

別名を表示する

PNAS-5, LYAR, Cell growth-regulating nucleolar protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-LYAR antibody [EPR14352-9] (AB182138)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LYAR antibody [EPR14352-9] (AB182138)

Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling LYAR with ab182138 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution. Counter stained with DAPI (blue).

Supplier Data

Immunoprecipitation - Anti-LYAR antibody [EPR14352-9] (AB182138)

Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab182138 at 1/60 dilution (Lane 1). Lane 2 : Negative control.
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-LYAR antibody [EPR14352-9] (ab182138)

Predicted band size: 44 kDa

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Supplier Data

Western blot - Anti-LYAR antibody [EPR14352-9] (AB182138)

All lanes:

Western blot - Anti-LYAR antibody [EPR14352-9] (ab182138) at 1/20000 dilution

Lane 1:

Ramos cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14352-9

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Tissue culture supernatant

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Lyar also known as the cell growth-regulating nucleolar protein is a zinc finger protein with a molecular mass of approximately 36 kDa. This protein is expressed primarily in the nucleolus although it can also be found in other sub-nuclear structures. Lyar binds to nucleic acids and proteins playing roles in nucleolus-related processes. Its expression is seen in various tissues but it is most pronounced in rapidly dividing cells such as those in fetal tissues and certain cancers.

Biological function summary

The Lyar protein plays a role in regulating cell growth and proliferation. It participates in ribosome biogenesis by interacting with pre-ribosomal RNA possibly aiding in the stabilization and processing of rRNA precursors. Lyar is not usually considered as part of a protein complex but its interactions with several other nucleolar proteins imply its involvement in complex regulatory networks. It influences the transcription of ribosomal RNA genes impacting protein synthesis rates and cell cycle progression.

Pathways

Lyar is involved in the modulation of pathways linked to cell proliferation and survival. Notably it affects the PI3K/AKT signaling pathway by interacting with nucleophosmin (NPM1) impacting cell growth and survival mechanisms. This collaboration can alter the response of cells to external growth signals influencing cellular responses to metabolic changes and stress.

Aberrant Lyar levels have been implicated in oncogenesis. Its overexpression has been noted in various cancers including leukemia and neuroblastoma linking it to uncontrolled cell division and tumor progression. In cancer Lyar interacts with tumorigenesis-associated proteins such as c-Myc enhancing their transcriptional activity and contributing to cancerous transformation. Understanding the role of Lyar in these disease processes remains essential for identifying potential therapeutic strategies and developing targeted interventions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (PubMed : 24495227). Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression (PubMed : 25092918). In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA (PubMed : 28686580). Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (PubMed : 25092918). Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity (PubMed : 31413131). In addition, inhibits NF-kappa-B-mediated expression of pro-inflammatory cytokines (PubMed : 31413131). Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (By similarity). Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal (By similarity).

See full target information LYAR

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8870 PubMed41053178

2025

Paraventricular nucleus CRH neurons regulate acute lung injury via sympathetic nerve-neutrophil axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Li,Tao Liu,Yang Wang,Xue-Mei Miao,Yi-Yu Xiong,Qian Zhao,Wei-Yun Shen,Fu-Hong Su,Kang Chen,Ru-Ping Dai

Cancer discovery 13:332-347 PubMed36259929

2022

A Dynamic rRNA Ribomethylome Drives Stemness in Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Fengbiao Zhou,Nesrine Aroua,Yi Liu,Christian Rohde,Jingdong Cheng,Anna-Katharina Wirth,Daria Fijalkowska,Stefanie Göllner,Michelle Lotze,Haiyang Yun,Xiaobing Yu,Caroline Pabst,Tim Sauer,Thomas Oellerich,Hubert Serve,Christoph Röllig,Martin Bornhäuser,Christian Thiede,Claudia Baldus,Michaela Frye,Simon Raffel,Jeroen Krijgsveld,Irmela Jeremias,Roland Beckmann,Andreas Trumpp,Carsten Müller-Tidow

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LYAR 抗体 [EPR14352-9]

Anti-LYAR antibody [EPR14352-9]

Immunocytochemistry/ Immunofluorescence - Anti-LYAR antibody [EPR14352-9] (AB182138)

Immunoprecipitation - Anti-LYAR antibody [EPR14352-9] (AB182138)

Western blot - Anti-LYAR antibody [EPR14352-9] (AB182138)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise