AB25377

Anti-Ly6g + Ly6c 抗体 [RB6-8C5]

Name: Anti-Ly6g + Ly6c 抗体 [RB6-8C5] (ab25377) | Abcam
Brand: Abcam Limited
SKU: AB25377
Price: 30000 JPY
Availability: InStock
Rating: 4 (5 reviews)

Anti-Ly6g + Ly6c antibody [RB6-8C5]

Lab Essentials
詳細を見る

(5 Reviews)

(267 Publications)

Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377) is a rat monoclonal antibody detecting Ly6c in Flow Cytometry, IHC-P, IHC-Fr. Suitable for Mouse.

- Over 190 publications - Trusted since 2005

Ly6G and Ly6C are surface markers commonly used to identify and differentiate subsets of myeloid cells, particularly neutrophils and monocytes, in immunological studies. Their expression patterns help researchers characterize inflammatory responses, track immune cell infiltration, and study disease mechanisms in models of autoimmunity, infection, and cancer

別名を表示する

Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g

2 Images

Immunohistochemistry (Frozen sections) - Anti-Ly6g + Ly6c antibody [RB6-8C5] (AB25377)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Ly6g + Ly6c antibody [RB6-8C5] (AB25377)

Immunohistochemistry (Frozen sections) analysis of Mouse frozen spleen tissue labeling Ly6g with ab25377. Right : Rat Anti-Mouse Ly-6G-UNLB followed by Mouse Anti-Rat IgG2b-FITC. Left : Isotype Control, Rat IgG2b-UNLB followed by Mouse Anti-Rat IgG2b-FITC. Blue - DAPI.

This image was generated using the ascites version of the product.

CiteAb

Immunohistochemistry - Anti-Ly6g + Ly6c antibody [RB6-8C5] (AB25377)

Immunohistochemistry-immunofluorescence using Anti-Ly6g + Ly6c antibody [RB6-8C5], ab25377. Publication image from Bertrand, L. et al., 2019, Nat Commun, 31043599. Legend direct from paper.

EcoHIV diminishes post-ischemic stroke NVU recovery. Mice were infected with EcoHIV and subjected to stroke as in Fig. 1. Brain sections were stained for laminin (a), ICAM-1 (b) and P-selectin (c) 24 h post stroke, and quantified for mean fluorescence index (MFI); n = 6 mice per group, 10 microvessels per mice, 2 independent experiments. d Time course of ICAM1 expression levels as quantified by western blotting in sham and ischemic stroke animals at days 1, 4, 7, and 14 post-stroke both in mock (M) and EcoHIV-infected (E) mice. Representative blots are shown, and quantified results from 5–12 samples per group are illustrated on the bar graphs. e Representative image (left) and quantified results (right) of infiltration of the infarct area by Lys6g immunoreactive cells (neutrophils) at 24 h post-ischemic stroke. The sections were also stained for MAP2 (neurons) and Hoechst (nuclei). Absence of MAP2 staining indicates infarct area. Data quantified from 6 mice per group, 2 independent experiments, 4 fields of view per mice at x20 magnification; Z stack images. Whiskers-box plots represent centerline median, with interquartile range and min-max whiskers. Source data are provided as a Source Data file. **p < 0.01; ***p < 0.001; unpaired t test. a–c Scale bars : 40 µm; e scale bar : 320 µm

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

RB6-8C5

アイソタイプ

IgG2b

軽鎖のタイプ

kappa

キャリアフリー

交差種

Mouse

アプリケーション

IHC-Fr, IHC-P, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

Full Length Protein corresponding to Mouse Ly6c1. Database link P0CW02

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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製品の詳細

Product Specifications

Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377) is a rat Monoclonal antibody and is validated for use in Flow Cyt, IHC-Fr, IHC-P in mouse samples.
Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377) specifically detects Ly6g +Ly6c (UniProt ID: P35461; Molecular weight: 9kDa) and is sold in a convenient trial size to enable initial testing (25 µg) and larger sizes for subsequent scaling up experiments (100 µg).

Quality and Validation

Abcam's high quality validation processes ensure Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377) has high sensitivity and specificity.
Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377) has been cited over 193 times in peer reviewed journals and is trusted by the scientific community.
Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377) has 5 independent reviews from customers.

Related Products

Antibody clone RB6-8C5 is also available pre-conjugated to a variety of labels for your convenience - PE, FITC, APC, PE/Cy7®, Biotin, redFluor™ 710, violetFluor™ 450 (ab24884, ab25024, ab25273, ab25514, ab25680, ab242065, ab253070).

This product was changed from ascites to tissue culture supernatant on 2nd March 2020. Lot numbers higher than GR3218400 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Tissue culture supernatant

バッファー組成

pH: 8.2 Constituents: 100% Borate buffered saline

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Ly6g and Ly6c also known as Ly-6G and Ly6G marker are glycosylphosphatidylinositol (GPI)-anchored cell surface proteins found primarily in the immune system. They participate in neutrophil surface differentiation with approximately 25-35 kDa being the mass of Ly6g. These targets are expressed mostly on neutrophils making them useful markers for identifying and isolating these immune cells. Researchers often use '8c5' and 'anti-Ly6g' antibodies for staining Ly6g along with Ly6c to study immune cell populations particularly in inflammatory responses.

Biological function summary

Ly6g and Ly6c facilitate the identification and migration of neutrophils to inflammation and infection sites. They are not considered part of a larger complex but play an important role in immune surveillance. They contribute to the immune system’s ability to target and respond to pathogens as Ly6g assists in the recruitment and activation of these immune cells. The modulation of these proteins can affect immune cell trafficking and tissue injury.

Pathways

The surface proteins Ly6g and Ly6c are heavily involved in innate immune response pathways and leukocyte transendothelial migration. Their expression and activity can alter the immune response and inflammation rates which is why their presence indicates active participation in immune response pathways. These markers also connect with other proteins involved in cell adhesion such as integrins highlighting their role in cellular interactions during immune responses.

Ly6g and Ly6c have implications in inflammatory diseases and certain cancers. Their expression levels may link to diseases like rheumatoid arthritis where they impact inflammatory cell migration. In cancer elevated levels of these proteins can associate with tumor-associated neutrophils which might influence tumor progression. Researchers use these markers often alongside other proteins like Gr-1 to explore the role of immune cells in these pathological conditions and potential therapeutic targets.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Ly6g

追加のターゲット

Ly6c1

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文献 (267)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1312 PubMed40885864

2025

A single-cell atlas of mouse central nervous system immune cells reveals unique infection-stage immune signatures during the progression of meningitis caused by Streptococcus suis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Jiang,Jikun Mei,Junhui Zhu,Yanyan Tian,Tong Wu,Ziheng Li,Zengshuai Wu,Tamim Abdelaal,Fengyang Li,Na Li,Liancheng Lei

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e11873 PubMed40832885

2025

Succinate Dehydrogenase Subunit A (SDHA) Mediated Microglia Extracellular Traps Formation Participating in Cerebral Ischemic Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Zhao,Tao Li,Meijuan Dang,Ye Li,Jialiang Lu,Ziwei Lu,Zhiyang Chen,Qiao Huang,Yujie Chen,Yang Yang,Yuxuan Feng,Xiaoya Wang,Yating Jian,Heying Wang,Yingying Guo,Lei Zhang,Yu Jiang,Songhua Fan,Shengxi Wu,Hong Fan,Fang Kuang,Guilian Zhang

Journal of inflammation (London, England) 22:33 PubMed40797324

2025

Hydroxychloroquine reduces metastatic tumor burden in pancreatic adenocarcinoma through myeloperoxidase inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Britney Niemann,Pavan Rao,Quinn Hopen,Kaitlyn Landreth,Angisha Basnet,Werner Geldenhuys,Tracy W Liu,Brian A Boone

Scientific reports 15:28976 PubMed40775512

2025

Identification and validation of hub genes related to neutrophil extracellular traps-mediated cell damage and immune recruitment during abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanlong Lu,Heng Wang,Maolin Qiao,Runze Chang,Jinshan Chen,Lizheng Li,Keyi Fan,Sheng Yan,Ruijing Zhang,Honglin Dong

Pharmaceuticals (Basel, Switzerland) 18: PubMed40573254

2025

- Signaling Drives Fibrocyte-Mediated Pulmonary Fibrosis: Mechanistic Insights and Therapeutic Potential.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Lu,Gaoming Wang,Xiangzhe Yang,Jing Luo,Haitao Ma,Liangbin Pan,Yu Yao,Kai Xie

Cell death discovery 11:279 PubMed40527896

2025

Roles of ACSL4/GPX4 and FSP1 in oxalate-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Keng Ye,Ruilong Lan,Zhimin Chen,Kunmei Lai,Yankun Song,Guoping Li,Huabin Ma,Hong Chen,Yanfang Xu

Science advances 11:eadt6632 PubMed40397747

2025

Astrocyte-derived CCL5-mediated CCR5 neutrophil infiltration drives depression pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Yao,Si-Yuan Jiang,Ying-Ying Jiao,Zhi-Yong Zhou,Zhu Zhu,Cong Wang,Ke-Zhong Zhang,Teng-Fei Ma,Gang Hu,Ren-Hong Du,Ming Lu

Journal of neuroinflammation 22:131 PubMed40380229

2025

Spatiotemporal dynamic changes of meningeal microenvironment influence meningeal lymphatic function following subarachnoid hemorrhage: from inflammatory response to tissue remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Bingrui Zhu,Changming Liu,Ming Luo,Jiarui Chen,Sixuan Tian,Tiantong Zhan,Yibo Liu,Haocheng Zhang,Zhen Wang,Jianmin Zhang,Yuanjian Fang,Sheng Chen,Xiaoyu Wang

Nature communications 16:3975 PubMed40295483

2025

Resistance to anti-LAG-3 plus anti-PD-1 therapy in head and neck cancer is mediated by Sox9+ tumor cells interaction with Fpr1+ neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Wang,Maosheng Cheng,Shuang Chen,Caihua Zhang,Rongsong Ling,Shuqing Qiu,Ke Chen,Bin Zhou,Qiuli Li,Wenbin Lei,Demeng Chen

Cell death & disease 16:313 PubMed40251174

2025

Regulation of neutrophil migration in acute pulmonary inflammation by extraneuronal α1 gamma-aminobutyric acid receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Anika Fuhr,Robin Roediger,Mariana Simelitidis,Jutta Gamper-Tsigaras,Markus Templin,Michael Kormann,Bernd Antkowiak,Uwe Rudolph,David Köhler,Peter Rosenberger,Kristian-Christos Ngamsri,Franziska M Konrad

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ly6g + Ly6c 抗体 [RB6-8C5]

Anti-Ly6g + Ly6c antibody [RB6-8C5]

Immunohistochemistry (Frozen sections) - Anti-Ly6g + Ly6c antibody [RB6-8C5] (AB25377)

Immunohistochemistry - Anti-Ly6g + Ly6c antibody [RB6-8C5] (AB25377)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (267)

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Abcam product promise