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リコンビナントRabMAb

Anti-LY6E/SCA-2 抗体 [EPR26038-105] (ab300399)

Submit a review Submit a question References (1)
Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
  • Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
  • Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26038-105] to LY6E/SCA-2
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-LY6E/SCA-2 antibody [EPR26038-105]
    LY6E/SCA-2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26038-105] to LY6E/SCA-2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: IP

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human tonsil tissue lysate, human ovary tissue lysate. IHC-P: Human breast and ovarian carcinoma, human gastric adenocarcinoma.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR26038-105

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300399の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
特記事項
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
追加情報
Is unsuitable for IP.

ターゲット情報

  • 組織特異性

    Widely expressed, predominantly in liver, kidney, ovary, spleen and peripheral blood Leukocytes.

  • 配列類似性

    Contains 1 UPAR/Ly6 domain.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Ly-6E antibody
      • LY6E antibody
      • LY6E_HUMAN antibody
      • Lymphocyte antigen 6E antibody
      • Retinoic acid-induced gene E protein antibody
      • RIG-E antibody
      • SCA-2 antibody
      • Stem cell antigen 2 antibody
      • Thymic shared antigen 1 antibody
      • TSA-1 antibody
      see all

    画像

    • Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
      Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
      All lanes : Anti-LY6E/SCA-2 antibody [EPR26038-105] (ab300399) at 1/1000 dilution

      Lane 1 : Human tonsil tissue lysate
      Lane 2 : Human pancreas tissue lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 13 kDa
      Observed band size: 13 kDa


      Exposure time: 48 seconds


      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Negative control: pancreas(PMID: 25862760).

    • Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
      Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
      All lanes : Anti-LY6E/SCA-2 antibody [EPR26038-105] (ab300399) at 1/1000 dilution

      Lane 1 : Human ovary tissue lysate
      Lane 2 : Human thymus tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 13 kDa
      Observed band size: 13 kDa


      Exposure time: 8 seconds


      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)

      Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling LY6E/SCA-2 with ab300399 at 1/500 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Cytoplasmic staining on human breast carcinoma is observed (PMID: 25862760). The section was incubated with ab300399 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)

      Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labelling LY6E/SCA-2 with ab300399 at 1/500 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Cytoplasmic staining on human ovarian carcinoma is observed (PMID: 25862760). The section was incubated with ab300399 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)

      Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labelling LY6E/SCA-2 with ab300399 at 1/500 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Cytoplasmic staining on human gastric adenocarcinoma (PMID: 25862760). The section was incubated with ab300399 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)

      Immunohistochemical analysis of paraffin-embedded human pancreas tissue labelling LY6E/SCA-2 with ab300399 at 1/500 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control: almost no staining on human pancreas (PMID: 25862760). The section was incubated with ab300399 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

    • Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
      Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab300399 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300399 は 1 報の論文で使用されています。

    • Xu D  et al. PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection. Nature 619:819-827 (2023). PubMed: 37438530

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