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AB300399

Anti-LY6E/SCA-2 抗体 [EPR26038-105]

Anti-LY6E/SCA-2 antibody [EPR26038-105]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • 詳細を見る

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(4 Publications)

Anti-LY6E/SCA-2 antibody [EPR26038-105] (ab300399) is a rabbit monoclonal antibody detecting LY6E/SCA-2 in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

9804, RIGE, SCA2, TSA1, LY6E, Lymphocyte antigen 6E, Ly-6E, Retinoic acid-induced gene E protein, Stem cell antigen 2, Thymic shared antigen 1, RIG-E, SCA-2, TSA-1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcin tissue labeling LY6E/SCA-2 with ab300399 at 1/500 (0.956 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on human ovarian carcinoma (PMID : 25862760).The section was incubated with ab300399 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)

Immunohistochemical analysis of paraffin-embedded Human breast carcino tissue labeling LY6E/SCA-2 with ab300399 at 1/500 (0.956 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on human breast carcinoma (PMID : 25862760).The section was incubated with ab300399 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)

Immunohistochemical analysis of paraffin-embedded Human gastric adenoc tissue labeling LY6E/SCA-2 with ab300399 at 1/500 (0.956 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on human gastric adenocarcinoma (PMID : 25862760).The section was incubated with ab300399 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling LY6E/SCA-2 with ab300399 at 1/500 (0.956 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : almost no staining on human pancreas (PMID : 25862760).The section was incubated with ab300399 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
  • WB

Supplier Data

Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (ab300399) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate

Lane 2:

Human pancreas tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 13 kDa

false

Exposure time: 48s

Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)
  • WB

Supplier Data

Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (AB300399)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (ab300399) at 1/1000 dilution

Lane 1:

Human ovary tissue lysate 20 µg

Lane 2:

Human thymus tissue lysate 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 13 kDa

false

Exposure time: 8s

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR26038-105

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Primary Antibodies

AB227637

Anti-Met (c-Met) antibody [SP44] - C-terminal

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - C-terminal (AB227637)

  • 5
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Primary Antibodies

AB209242

Anti-B7H4 antibody [EPR20236]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [EPR20236] (AB209242)

  • 0
  • 0
Primary Antibodies

AB134161

Anti-CD276 antibody [EPNCIR122]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-CD276 antibody [EPNCIR122] (AB134161)

  • 0
  • 0
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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製品の詳細

What is this antibody validated in?
Anti-LY6E/SCA-2 antibody [EPR26038-105] (ab300399) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of LY6E/SCA-2?
Anti-LY6E/SCA-2 [EPR26038-105] (ab300399) specifically detects a band for LY6E/SCA-2 (UniProt: Q16553) at a molecular weight of 13kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR26038-105] also available for your convenience: ab300399, Carrier free - ab300411

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

LY6E also known as SCA-2 is a membrane protein known for its role in immune response. With a mass of approximately 15 kDa LY6E is expressed on the cell surface in various tissues including the immune system and some epithelial cells. The protein belongs to the LY6 family which is characterized by the presence of a Ly6/uPAR domain playing a part in cellular signaling and host defense mechanisms.
Biological function summary

LY6E has a significant impact on immune modulation and viral infection processes. It interacts with other immune-related proteins and contributes to the regulation of T-cell proliferation and differentiation. While LY6E is not a part of a multi-protein complex itself its activity can modify the function of immune cells and potentially alter immune responses. This makes it an important player in the context of both adaptive and innate immunity.

Pathways

LY6E participates in key immune signaling pathways particularly those related to interferon signaling and antiviral defense. It influences these pathways by interacting with components such as interferon-gamma and other cytokines which regulate immune cell behavior and response. LY6E affects the STAT pathway by indirectly modulating the activity of STAT1 a critical protein in immune response highlighting its importance in orchestrating immune dynamics.

LY6E has been implicated in several viral infections and cancers. Its expression alters during viral infections such as influenza and HIV where it may play a role in enhancing or suppressing viral replication. LY6E's interaction with other proteins like STAT1 and various viral envelope proteins could contribute to immune escape or disease progression. Moreover aberrant regulation of LY6E levels has connections with cancer progression affecting tumor immunity and potentially making it a target for therapeutic interventions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

GPI-anchored cell surface protein that regulates T-lymphocytes proliferation, differentiation, and activation. Regulates the T-cell receptor (TCR) signaling by interacting with component CD3Z/CD247 at the plasma membrane, leading to CD3Z/CD247 phosphorylation modulation (By similarity). Restricts the entry of human coronaviruses, including SARS-CoV, MERS-CoV and SARS-CoV-2, by interfering with spike protein-mediated membrane fusion (PubMed : 32641482). Also plays an essential role in placenta formation by acting as the main receptor for syncytin-A (SynA). Therefore, participates in the normal fusion of syncytiotrophoblast layer I (SynT-I) and in the proper morphogenesis of both fetal and maternal vasculatures within the placenta. May also act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity (By similarity).. (Microbial infection) Promotes entry, likely through an enhanced virus-cell fusion process, of various viruses including HIV-1, West Nile virus, dengue virus and Zika virus (PubMed : 28130445). In contrast, the paramyxovirus PIV5, which enters at the plasma membrane, does not require LY6E (PubMed : 28130445, PubMed : 29610346). Mechanistically, adopts a microtubule-like organization upon viral infection and enhances viral uncoating after endosomal escape (PubMed : 28130445, PubMed : 30190477).
See full target information LY6E
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:11431 PubMed40180998

2025

LY6E as a new prognostic biomarker of multiple myeloma-related bone disease.

Applications

Unspecified application

Species

Unspecified reactive species

Min Shi,Jing Li,Jing Wang,Ye Yao,Xuxing Shen,Yuan Xia,Ji Xu

Cancer immunology, immunotherapy : CII 74:4 PubMed39487896

2024

Ly6E on tumor cells impairs anti-tumor T-cell responses: a novel mechanism of tumor-induced immune exclusion.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Hailin,Chen Yiting,Wu Yue,Li Lijun,Zhang Renlu,Chen Yunhan,Zhu Yanyang,Zhang Qiuyu

Journal of translational medicine 22:211 PubMed38419085

2024

A multi-dimensional approach to unravel the intricacies of lactylation related signature for prognostic and therapeutic insight in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Huixia Huang,Keji Chen,Yifei Zhu,Zijuan Hu,Yaxian Wang,Jiayu Chen,Yuxue Li,Dawei Li,Ping Wei

Nature 619:819-827 PubMed37438530

2023

PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Dijin Xu,Weiqian Jiang,Lizhen Wu,Ryan G Gaudet,Eui-Soon Park,Maohan Su,Sudheer Kumar Cheppali,Nagarjuna R Cheemarla,Pradeep Kumar,Pradeep D Uchil,Jonathan R Grover,Ellen F Foxman,Chelsea M Brown,Phillip J Stansfeld,Joerg Bewersdorf,Walther Mothes,Erdem Karatekin,Craig B Wilen,John D MacMicking
View all publications
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