Anti-LXR beta/NER 抗体

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LXR beta/NER antibody (AB28479)

ab28479 staining LXR beta/NER in C2C12 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2ug/ml) for 3 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/2000). Nuclei stained blue and F-actin stained red. Panel d shows the merged image

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LXR beta/NER antibody (AB28479)

ab28479 labelling LXR beta/NER (green) in the nuclear envelope of C2C12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

• WB

Supplier Data

Western blot - Anti-LXR beta/NER antibody (AB28479)

A 51 kDa band corresponding to LXR beta was observed across the cell lines and tissue tested.

All lanes:

Western blot - Anti-LXR beta/NER antibody (ab28479) at 2 µg/mL

Lane 1:

Hep G2 nuclear enriched extract at 30 µg

Lane 2:

PC-3 nuclear enriched extract at 30 µg

Lane 3:

3T3-L1 nuclear enriched extract at 30 µg

Lane 4:

Mouse Lung tissue extract at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/4000 dilution

Predicted band size: 50 kDa

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• WB

Supplier Data

Western blot - Anti-LXR beta/NER antibody (AB28479)

All lanes:

Western blot - Anti-LXR beta/NER antibody (ab28479) at 1/1000 dilution

Lane 1:

Rat liver cell lysate at 25 µg

Lane 2:

Mouse liver cell lysate at 25 µg

Lane 3:

HeLa cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit

Predicted band size: 50 kDa

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• WB

CiteAb

Western blot - Anti-LXR beta/NER antibody (AB28479)

Western Blotting using Anti-LXR beta/NER antibody, ab28479. Publication image from Singh, K. S. et al., 2020, Nat Commun, 31980600. Legend direct from paper.

LXR agonists lower iron content, reverse anti-inflammatory response, and improve Lm killing in S47 mouse macrophages.a Iron accumulation in P47 and S47 MDMs under different treatment conditions by Prussian blue staining. (n = 10 biological replicates, 3–4 fields per sample), Bars, 50 µm. b Iron levels in P47 and S47 MDMs under different treatment conditions measured by loss of calcein fluorescence. Gray histogram = unstained control. (representative from 10 biological replicates). c P47 and S47 mouse splenocytes pretreated with DMSO, GW3965, or T0901317 analyzed for CD4 + , CD25 + and FoxP3-Hi Treg at baseline or in the presence of Lm infection. d Relative protein levels in P47 and S47 mouse MDMs measured by immunoblot after 24 h of indicated treatments. e S47 & P47 mice infected with Lm are treated with PBS or LXR agonist T0901317 and monitored daily from day 2 post infection for survival (n = 5–6 biological replicates) f Spleens of P47 and S47 mice at 9 days post infection. scale = 4 cm. g Bacterial load in different organs at the experimental endpoint measured as CFU/gm (n = 5–6 biological replicates with three technical replicates), compares changes in bacterial load in P47 & S47 mice following T0901317 treatment. Error bars represent means ± s.e.m. P < 0.01, *P < 0.05, ns—not significant; by unpaired Student’s t test, relative to P47 mice. h Arginase activity, i NOS activity, and j Bacterial CFU, measured in P47 (white) and S47 (black) under indicated conditions (n = 5 biological and three technical replicates). Error bars represent means ± s.e.m. ***P < 0.001, **P < 0.01, ns—not significant; by unpaired Student’s t test, relative to P47 mice or to S47 mice where indicated. Source data are provided as a Source Data file.

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