AB24362

Anti-LXR alpha + LXR beta 抗体

Name: Anti-LXR alpha + LXR beta 抗体 (ab24362) | Abcam
Brand: Abcam Limited
SKU: AB24362
Price: 78000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Anti-LXR alpha + LXR beta antibody

(2 Reviews)

(8 Publications)

Goat Polyclonal LXR alpha antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human NR1H3.

別名を表示する

LXRA, NR1H3, Oxysterols receptor LXR-alpha, Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3

3 Images

Flow Cytometry (Intracellular) - Anti-LXR alpha + LXR beta antibody (AB24362)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LXR alpha + LXR beta antibody (AB24362)

Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton, followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-LXR alpha + LXR beta antibody (AB24362)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LXR alpha + LXR beta antibody (AB24362)

Immunocytochemical/Immunofluorescent analysis of paraformaldehyde-fixed, 0.15% triton permeabilized A549 cells labelling LXR alpha + LXR beta with ab24362 at 10μg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Supplier Data

Western blot - Anti-LXR alpha + LXR beta antibody (AB24362)

All lanes:

Western blot - Anti-LXR alpha + LXR beta antibody (ab24362) at 1 µg/mL

Lane 1:

Human liver lysate in RIPA buffer at 35 µg

Lane 2:

Human kidney lysate in RIPA buffer at 35 µg

Predicted band size: 51 kDa

true

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB, Flow Cyt (Intra)

applications

免疫原

Synthetic Peptide within Human NR1H3. The exact immunogen used to generate this antibody is proprietary information.

Q13133

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Liver X Receptors (LXRs) including LXR alpha and LXR beta are nuclear receptors that function as transcription factors. These proteins with LXR alpha weighing approximately 51 kDa regulate the expression of genes involved in lipid metabolism and homeostasis. LXR alpha is mainly expressed in liver adipose tissue macrophages and the intestine whereas LXR beta shows a more ubiquitous expression pattern across tissues. Both receptors are activated by oxysterols which are oxidized derivatives of cholesterol.

Biological function summary

The liver X receptors play a significant role in regulating cholesterol and fatty acid homeostasis. LXR alpha and LXR beta do not function in isolation; they form heterodimers with Retinoid X Receptors (RXRs) to bind specific DNA sequences in target genes. Upon ligand activation they modulate the expression of ATP-binding cassette transporters like ABCA1 and ABCG1 which facilitate cholesterol efflux from cells to high-density lipoprotein (HDL) particles. The modulation of these genes is essential for maintaining cholesterol levels and preventing lipid accumulation in cells.

Pathways

LXRs integrate into the complex network of cholesterol and lipid metabolic pathways. They are key regulators of the cholesterol efflux pathway impacting the homeostasis of cholesterol through their interaction with RXR. In addition they influence the inflammatory response pathways by modulating the expression of inflammatory cytokines in macrophages. This function positions LXRs at the intersection of lipid metabolism and immune responses interlinking with proteins such as peroxisome proliferator-activated receptors (PPARs) that also play a role in these pathways.

LXRs have been linked to conditions such as atherosclerosis and metabolic syndrome. By regulating cholesterol homeostasis LXRs contribute to plaque formation in arteries an important feature of atherosclerosis. Furthermore their role in inflammation suggests LXRs also influence metabolic syndrome and its related disorders. In these contexts LXRs interact with proteins like apolipoprotein E (ApoE) which is vital for cholesterol transport and clearance and C-reactive protein (CRP) which is involved in the inflammatory response. This connection highlights their potential as therapeutic targets in these diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed : 19481530, PubMed : 25661920, PubMed : 37478846). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES (PubMed : 37478846). LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8 (PubMed : 19481530). Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis (By similarity). Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators (By similarity).

See full target information NR1H3

追加のターゲット

NR1H2

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 15: PubMed38068770

2023

Theabrownin from Wuniuzao Dark Tea Regulates Hepatic Lipid Metabolism and Gut Microbiota in Mice Fed High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Xu,Jiangcheng Ye,Mingxiu Gong,Yifan Zhang,Yiwei Yuan,Jin Zhao

Oncogene 41:2685-2695 PubMed35379924

2022

27-hydroxycholesterol linked high cholesterol diet to lung adenocarcinoma metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xingkai Li,Hengchi Chen,Lizhen Zhang,Li Chen,Wei Wei,Shugeng Gao,Qi Xue,Yue Li,Bing Wang,Jiagen Li,Yushun Gao,Yanliang Lin

Clinica chimica acta; international journal of cli 499:134-141 PubMed31526774

2019

Circulating ectodysplasin A is a potential biomarker for nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Yang,Wenjing Zhou,Jinzhou Zhu,Yue Wu,Liqian Xu,Yuming Wang,Qin Zhang,Yunmei Yang

American journal of translational research 11:1498-1509 PubMed30972177

2019

Fetuin B aggravates liver X receptor-mediated hepatic steatosis through AMPK in HepG2 cells and mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Zhou,Ji Yang,Jinzhou Zhu,Yuming Wang,Yue Wu,Liqian Xu,Yunmei Yang

Proteomics. Clinical applications 10:750-9 PubMed26934842

2016

Aqueous Terminalia arjuna extract modulates expression of key atherosclerosis-related proteins in a hypercholesterolemic rabbit: A proteomic-based study.

Applications

Unspecified application

Species

Unspecified reactive species

Riyaz Ahmad Rather,Vivek Singh Malik,Dimple Trikha,Owais Bhat,Veena Dhawan

Journal of lipid research 55:1944-58 PubMed25030662

2014

Lxr-driven enterocyte lipid droplet formation delays transport of ingested lipids.

Applications

Unspecified application

Species

Unspecified reactive species

Lourdes Cruz-Garcia,Amnon Schlegel

The Journal of biological chemistry 288:8505-18 PubMed23362264

2013

Mechanisms of action of hormone-sensitive lipase in mouse Leydig cells: its role in the regulation of the steroidogenic acute regulatory protein.

Applications

Species

Mouse

Pulak R Manna,Joëlle Cohen-Tannoudji,Raymond Counis,Charles W Garner,Ilpo Huhtaniemi,Fredric B Kraemer,Douglas M Stocco

Cell 142:320-32 PubMed20655472

2010

SIRT1 suppresses beta-amyloid production by activating the alpha-secretase gene ADAM10.

Applications

Species

Mouse

Gizem Donmez,Diana Wang,Dena E Cohen,Leonard Guarente

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LXR alpha + LXR beta 抗体

Anti-LXR alpha + LXR beta antibody

Flow Cytometry (Intracellular) - Anti-LXR alpha + LXR beta antibody (AB24362)

Immunocytochemistry/ Immunofluorescence - Anti-LXR alpha + LXR beta antibody (AB24362)

Western blot - Anti-LXR alpha + LXR beta antibody (AB24362)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (8)

代替製品

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise