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Anti-LXR alpha 抗体 [PPZ0412] (ab41902)

Reviews (12)Q&A (17)References (81)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (ab41902)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)
  • Flow Cytometry - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)

Key features and details

  • Mouse monoclonal [PPZ0412] to LXR alpha
  • Suitable for: ICC/IF, ELISA, IP, ChIP, GSA, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-LXR alpha antibody [PPZ0412]
    LXR alpha 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [PPZ0412] to LXR alpha

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, ELISA, IP, ChIP, GSA, IHC-P, Flow Cytmore details
    適用なし: WB

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment corresponding to Human LXR alpha aa 164-447.
    Database link: Q13133

  • ポジティブ･コントロール

    • Rat hepatocyte and kupffer cell. Rat spleen macrophage.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab41902の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF (1)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ChIP (1)
Use at an assay dependent concentration.
GSA
Use at an assay dependent concentration.
EMSA
Use at an assay dependent concentration.
IHC-P (2)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
特記事項
ICC/IF
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ChIP
Use at an assay dependent concentration.
GSA
Use at an assay dependent concentration.
EMSA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
追加情報
Is unsuitable for WB.

ターゲット情報

  • 機能

    Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.

  • 組織特異性

    Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.

  • 配列類似性

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Liver X receptor alpha antibody
    • LXR a antibody
    • LXRA antibody
    • NR1H3 antibody
    • NR1H3_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR-alpha antibody
    • RLD 1 antibody
    • RLD1 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (ab41902)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (ab41902)This image is courtesy of an abreview submitted by Rudolf Jung, University of Regensburg.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling LXR alpha with ab41902 at 1/100 dilution. Tissue was fixed with formaldehyde and permeabilized with Tween 20. Heat mediated antigen retrieval was performed using TRIS-EDTA Buffer. Tissue sections were incubated with Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) for 30 minutes at 20°C in diluent from DAKO. An undiluted HRP conjugated horse anti-mouse secondary antibody was used.

    This image was generated using the ascites version of the product.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)

    ab41902 staining LXR alpha in rat spleen macrophage cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)

    ab41902 staining LXR alpha in Rat Hepatocyte and Kupffer cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)
    Flow Cytometry - Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902)

    Overlay histogram showing HepG2 cells stained with ab41902 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41902, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

参考文献 (81)

ab41902 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab41902 は 81 報の論文で使用されています。

  • Guo BC  et al. Di-(2-ethylhexyl) Phthalate Limits the Lipid-Lowering Effects of Simvastatin by Promoting Protein Degradation of Low-Density Lipoprotein Receptor: Role of PPARγ-PCSK9 and LXRα-IDOL Signaling Pathways. Antioxidants (Basel) 12:N/A (2023). PubMed: 36830035
  • Sun X  et al. TRIB1 regulates liver regeneration by antagonizing the NRF2-mediated antioxidant response. Cell Death Dis 14:372 (2023). PubMed: 37355685
  • Chinetti G  et al. Diabetes-Induced Changes in Macrophage Biology Might Lead to Reduced Risk for Abdominal Aortic Aneurysm Development. Metabolites 12:N/A (2022). PubMed: 35208203
  • Lin CY  et al. The Citrus Flavonoid Nobiletin Downregulates Angiopoietin-like Protein 3 (ANGPTL3) Expression and Exhibits Lipid-Modulating Effects in Hepatic Cells and Adult Zebrafish Models. Int J Mol Sci 23:N/A (2022). PubMed: 36293338
  • Zhang J  et al. Low expression of NR1H3 correlates with macrophage infiltration and indicates worse survival in breast cancer. Front Genet 13:1067826 (2022). PubMed: 36699456
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 29 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LXR alpha antibody [PPZ0412]

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: trilogy
Permeabilization
No
Specification
liver
Blocking step
leica protein block as blocking agent for 15 minute(s) · Concentration: 0.4% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 21 2023

Western blot abreview for Anti-LXR alpha antibody [PPZ0412] - ChIP Grade

 Good
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (monocyte-derived macrophage)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
100000 cells
Specification
monocyte-derived macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jun 29 2015

Western blot abreview for Anti-LXR alpha antibody [PPZ0412] - ChIP Grade

 Excellent
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Loading amount
30 µg
Specification
HepG2 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Dongil Kim

Verified customer

投稿 Apr 08 2013

Western blot abreview for Anti-LXR alpha antibody [PPZ0412] - ChIP Grade

 Good
Abreviews
This product is known to not work in this application or species.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (lung)
Loading amount
30 µg
Specification
lung
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Jihun Lee

Verified customer

投稿 Jan 10 2013

Western blot abreview for Anti-LXR alpha antibody [PPZ0412] - ChIP Grade

 Good
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Mono-mac 6)
Loading amount
50 µg
Specification
Mono-mac 6
Treatment
TO-901317
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Elizabeth Kilby

Verified customer

投稿 Dec 19 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-LXR alpha antibody [PPZ0412] - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (THP1 monocyte-derived macrophages)
Specification
THP1 monocyte-derived macrophages
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Elizabeth Kilby

Verified customer

投稿 Oct 17 2012

Western blot abreview for Anti-LXR alpha antibody [PPZ0412] - ChIP Grade

 Excellent
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat liver tissue)
Loading amount
120 µg
Specification
Rat liver tissue
Treatment
ovariectomy
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
5% milk in TBS-T(0.1%) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Smitha Koshy

Verified customer

投稿 May 21 2012

Question

Thank you for your offer to replace the antibody. I would prefer to take ab106464 as it has been tested with rat liver lysate.It will be extremely helpful if you can send it along with your next shipment to the distributor.
I'm almost on the verge of winding up my work, so kindly help me with a speedy supply.

Read More
Answer

As requested, one unit of ab106464 has been added to the next order to be shipped to our distributor.
I wish all the best with your reseach work.

Read More

Question

Product code: 41902
Lot number: GR9889-6
Inquiry: I had purchased one vial of ab41902, LXR alpha antibodylot no: GR9889-6 I performed a western blot with rat liver lysate(nuclear lysate). Homogenising buffer: Buffer A: 10mM HEPES, 1.5mM MgCl2, 10mM KCl, 0.05%NP40, with protease inhibitors.
Homogenate was centrifuged at 800G for 10 min.
Nuclear pellet was washed in same buffer and then suspended in RIPA buffer with protease inhibitors.
Homogenate was briefly sonicated for 3 pulses of 5 sec each.
Centrifuged at 24,000 G for 20 min.
Supernatant was used as the nuclear fraction.
Running conditions: 10% SDS-PAGE with 4% stacking. 100V for 20 min, 200V for 40 min.
Amount of protein loaded per well: 50µg
Blocking: 5% milk in TBST(0.1%)- 1hr at RT.
Wash with TBST(0.1%)- 3X 5 min
Primary antibody: 1:1000 in 2.5% BSA TBST(0.1%)-16 hrs at 4°C.
Wash with TBST(0.1%)- 3X 5 min
Secondary antibody: 1:1000 anti mouse IgG in 5% milk in TBST(0.1%)- 1 hr at RT.
Wash with TBST(0.1%)- 3X 5 min Developed using ECL West pico chemiluminescent substrate.
Exposure time: 15 sec Observed bands: Prominent double bands at 42 and 52 KDa.
The predicted molecular weight is around 50KDa.
Does this antibody cross react with LXR beta as well?. Which among the 2 doublet bands is LXR?

Read More
Answer

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. ab41902 is specific to LXR alpha and should not cross-react with LXR beta.
Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.
I apologize for the inconvenience and am pleased to offer you in compensation a credit note, or a refund or a free of charge replacement with an alternative anti-LXR alpha such as ab3585 (www.abcam.com/ab3585) or ab106464 (www.abcam.com/ab106464).
Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Read More

Question

Customer kindly called as they were experiencing high non specific background in western blot on Bone Marrow-Derivated Macrophage samples.

Read More
Answer

Thank you for contacting us.

I have been in contact with the lab on your behalf requesting the blocking peptide for ab41902. Unfortunately this is not available. I am very sorry for any inconvenience and will continue to work with you to get the best results possible with this product.

Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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1-10 of 29 Abreviews or Q&A

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