Anti-LXR alpha 抗体 (ab3585)
Key features and details
- Rabbit polyclonal to LXR alpha
- Suitable for: ICC, IHC-FoFr, ICC/IF
- Reacts with: Mouse, Rat, Human, Chinese hamster
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LXR alpha antibody
LXR alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LXR alpha -
由来種
Rabbit -
特異性
Detects recombinant Human LXR alpha. This antibody does not detect recombinant Human LXR beta. -
アプリケーション
適用あり: ICC, IHC-FoFr, ICC/IFmore details
適用なし: WB -
種交差性
交差種: Mouse, Rat, Human, Chinese hamster
交差が予測される動物種: Cow -
免疫原
Synthetic peptide corresponding to Human LXR alpha aa 318-329.
Sequence:DFSYNREDFAKA
(Peptide available asab5021) -
ポジティブ・コントロール
- ICC: MCF7, HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
Liver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of genesis and metabolism of cholesterols and bile acids. LXRs form heterodimeric complexes with the retinoic acid receptors (RARs) once activated by their respective oxysterol ligands. Two known isoforms, alpha and beta, are differentially expressed; the alpha isoform predominantly expressed in liver, whereas beta isoform expression has been shown to be ubiquitous. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3585の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
1/50 - 1/500.
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EMSA |
Use at an assay dependent concentration.
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Gel Shift Assay |
Use at an assay dependent concentration.
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 22836489
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ICC/IF | (2) |
Use at an assay dependent concentration.
|
特記事項 |
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ICC
1/50 - 1/500. |
EMSA
Use at an assay dependent concentration. |
Gel Shift Assay
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 22836489 |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. -
組織特異性
Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. -
配列類似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 507176 Cow
- Entrez Gene: 10062 Human
- Entrez Gene: 22259 Mouse
- Entrez Gene: 58852 Rat
- Omim: 602423 Human
- SwissProt: Q5E9B6 Cow
- SwissProt: Q13133 Human
- SwissProt: Q9Z0Y9 Mouse
see all -
別名
- Liver X receptor alpha antibody
- LXR a antibody
- LXRA antibody
see all
画像
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Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (20)
ab3585 は 20 報の論文で使用されています。
- Han W et al. Danlou Recipe promotes cholesterol efflux in macrophages RAW264.7 and reverses cholesterol transport in mice with hyperlipidemia induced by P407. BMC Complement Med Ther 23:445 (2023). PubMed: 38066464
- Li T et al. The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRa and PPARa. J Cell Mol Med 24:3384-3398 (2020). PubMed: 31981312
- Yu W et al. Activation of LXRß Signaling in the Amygdala Confers Anxiolytic Effects Through Rebalancing Excitatory and Inhibitory Neurotransmission upon Acute Stress. Neurotherapeutics 17:1253-1270 (2020). PubMed: 32297184
- Xu D et al. SIRT2 plays a novel role on progesterone, estradiol and testosterone synthesis via PPARs/LXRa pathways in bovine ovarian granular cells. J Steroid Biochem Mol Biol 185:27-38 (2019). PubMed: 30009951
- Yin J et al. The fucoidan from the brown seaweed Ascophyllum nodosum ameliorates atherosclerosis in apolipoprotein E-deficient mice. Food Funct 10:5124-5139 (2019). PubMed: 31364648