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Anti-LXR alpha + LXR beta 抗体 (ab24362)

Reviews (2)Q&A (2)References (7)
Immunocytochemistry/ Immunofluorescence - Anti-LXR alpha + LXR beta antibody (ab24362)
  • Flow Cytometry (Intracellular) - Anti-LXR alpha + LXR beta antibody (ab24362)
  • Western blot - Anti-LXR alpha + LXR beta antibody (ab24362)

Key features and details

  • Goat polyclonal to LXR alpha + LXR beta
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-LXR alpha + LXR beta antibody
    LXR alpha + LXR beta 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to LXR alpha + LXR beta

  • 由来種

    Goat

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IF, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish

  • 免疫原

    Peptide with sequence (C)RLQDKKLPPLLSEI, from the C-terminus of the human protein sequences according to NP_005684; NP_009052.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: Human kidney and liver lysate; Flow Cyt (intra): A549 cells; ICC/IF: A549 cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab24362の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 10 µg/ml.
WB (2)
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

1 hour primary incubation is recommended for this product.
特記事項
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

1 hour primary incubation is recommended for this product.

ターゲット情報

  • 関連性

    LXR alpha and LXR beta are orphan receptors. Interaction with RXR shifts RXR from its role as a silent DNA binding partner to an active ligand binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4 type response elements characterized by direct repeats of two similar hexanuclotide half sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis. LXR alpha: Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. LXR beta: Ubiquitously expressed.

  • 細胞内局在

    Nuclear

  • 参照データベース

    see all

  • 別名

    • Liver X receptor alpha antibody
    • Liver X receptor beta antibody
    • LXR a antibody
    • LXR b antibody
    • LXRalpha antibody
    • LXRbeta antibody
    • NR1H2 antibody
    • NR1H3 antibody
    • Nuclear orphan receptor LXR alpha antibody
    • Nuclear orphan receptor LXR beta antibody
    • Nuclear receptor subfamily 1 group H member 2 antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR beta antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-LXR alpha + LXR beta antibody (ab24362)
    Immunocytochemistry/ Immunofluorescence - Anti-LXR alpha + LXR beta antibody (ab24362)

    Immunocytochemical/Immunofluorescent analysis of paraformaldehyde-fixed, 0.15% triton permeabilized A549 cells labelling LXR alpha + LXR beta with ab24362 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).

    Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

  • Flow Cytometry (Intracellular) - Anti-LXR alpha + LXR beta antibody (ab24362)
    Flow Cytometry (Intracellular) - Anti-LXR alpha + LXR beta antibody (ab24362)

    Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton, followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

  • Western blot - Anti-LXR alpha + LXR beta antibody (ab24362)
    Western blot - Anti-LXR alpha + LXR beta antibody (ab24362)
    All lanes : Anti-LXR alpha + LXR beta antibody (ab24362) at 1 µg/ml

    Lane 1 : Human liver lysate in RIPA buffer
    Lane 2 : Human kidney lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 51 kDa

参考文献 (7)

ab24362 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab24362 は 7 報の論文で使用されています。

  • Li X  et al. 27-hydroxycholesterol linked high cholesterol diet to lung adenocarcinoma metastasis. Oncogene 41:2685-2695 (2022). PubMed: 35379924
  • Zhou W  et al. Fetuin B aggravates liver X receptor-mediated hepatic steatosis through AMPK in HepG2 cells and mice. Am J Transl Res 11:1498-1509 (2019). PubMed: 30972177
  • Yang J  et al. Circulating ectodysplasin A is a potential biomarker for nonalcoholic fatty liver disease. Clin Chim Acta 499:134-141 (2019). PubMed: 31526774
  • Rather RA  et al. Aqueous Terminalia arjuna extract modulates expression of key atherosclerosis-related proteins in a hypercholesterolemic rabbit: A proteomic-based study. Proteomics Clin Appl 10:750-9 (2016). PubMed: 26934842
  • Cruz-Garcia L & Schlegel A Lxr-driven enterocyte lipid droplet formation delays transport of ingested lipids. J Lipid Res 55:1944-58 (2014). PubMed: 25030662
  • Manna PR  et al. Mechanisms of action of hormone-sensitive lipase in mouse Leydig cells: its role in the regulation of the steroidogenic acute regulatory protein. J Biol Chem 288:8505-18 (2013). WB ; Mouse . PubMed: 23362264
  • Donmez G  et al. SIRT1 Suppresses beta-Amyloid Production by Activating the alpha-Secretase Gene ADAM10. Cell 142:320-332 (2010). WB ; Mouse . PubMed: 20655472

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-LXR alpha + LXR beta antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rabbit Tissue lysate - whole (heart)
Loading amount
40 µg
Specification
heart
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 06 2012

Western blot abreview for Anti-LXR alpha + LXR beta antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Cow Cell lysate - whole cell (muscle cells)
Loading amount
40 µg
Specification
muscle cells
Treatment
with LXR agonist for 24 hours
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Fabien Gosselet

Verified customer

投稿 Nov 29 2010

Question

I would like to test this antibody in rabbit.

Read More
Answer

I am very pleased to hear you would like to accept our offer and test ab24362 in rabbit. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forrabbit in WBand include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question

First, we would like to make out if you have antibodes for LXR alpha and LXR beta separately. i mean when we do Western blot we can tell them from each other rather than take them together.

Read More
Answer

Thank you for your enquiry. We have the following antibodies which may be of interest to you, please take a look at the online datasheets to see if there are applicable for your experiments (both have been tested in Western blotting): ab3585 - Rabbit polyclonal to LXR alpha ab24361 - Goat polyclonal to LXR beta If you have any additional questions, please contact us again.

Read More

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