Anti-Lumican 抗体 [EPR8898(2)] (ab168348)
![Western blot - Anti-Lumican antibody [EPR8898(2)] (ab168348)](https://www.abcam.co.jp/ps/products/168/ab168348/Images/ab168348-168804-anti-lumican-antibody-epr88982-western-blot.jpg "Western blot - Anti-Lumican antibody [EPR8898(2)] (ab168348)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8898(2)] to Lumican
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Lumican antibody [EPR8898(2)]
Lumican 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8898(2)] to Lumican -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human Lumican aa 250-350. The exact sequence is proprietary.
Database link: P51884 -
ポジティブ･コントロール
- WB: Saos-2, fetal skin, fetal heart, Jurkat, Y79 and HEK-293T lysates. IHC-P: Human kidney and Human skin tissues. ICC/IF: Saos-2 cells. Flow Cyt (intra): HEK-293T cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8898(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168348の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).
|
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| IHC-P | (6) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| ICC/IF | (3) |
1/250 - 1/500.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/250 - 1/500. |
ターゲット情報
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組織特異性
Cornea and other tissues. -
配列類似性
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
発生段階
Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan. -
翻訳後修飾
Sulfated on tyrosine residue(s). -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 4060 Human
- Entrez Gene: 17022 Mouse
- Entrez Gene: 81682 Rat
- Omim: 600616 Human
- SwissProt: P51884 Human
- SwissProt: P51885 Mouse
- SwissProt: P51886 Rat
- Unigene: 406475 Human
see all -
別名
- Keratan sulfate proteoglycan lumican antibody
- KSPG lumican antibody
- LDC antibody
see all
画像
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Western blot - Anti-Lumican antibody [EPR8898(2)] (ab168348)All lanes : Anti-Lumican antibody [EPR8898(2)] (ab168348) at 1/1000 dilution
Lane 1 : Saos-2 lysate
Lane 2 : Fetal skin lysate
Lane 3 : Fetal heart lysate
Lane 4 : Jurkat lysate
Lane 5 : Y79 lysate
Lane 6 : 293T lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR8898(2)] (ab168348)](https://www.abcam.co.jp/ps/products/168/ab168348/Images/ab168348-168811-anti-lumican-antibody-epr88982-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR8898(2)] (ab168348)Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Lumican with ab168348 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Lumican antibody [EPR8898(2)] (ab168348)](https://www.abcam.co.jp/ps/products/168/ab168348/Images/ab168348-168812-anti-lumican-antibody-epr88982-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Lumican antibody [EPR8898(2)] (ab168348)Immunofluorescent analysis of Saos-2 cells labeling Lumican with ab168348 at 1/250.
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![Flow Cytometry (Intracellular) - Anti-Lumican antibody [EPR8898(2)] (ab168348)](https://www.abcam.co.jp/ps/products/168/ab168348/Images/ab168348-168813-anti-lumican-antibody-epr88982-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Lumican antibody [EPR8898(2)] (ab168348)Intracellular flow cytometrical analysis of permeabilized 293T cells with ab168348 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR8898(2)] (ab168348)](https://www.abcam.co.jp/ps/products/168/ab168348/Images/ab168348-168810-anti-lumican-antibody-epr88982-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR8898(2)] (ab168348)Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Lumican with ab168348 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-Lumican antibody [EPR8898(2)] (ab168348)](https://www.abcam.co.jp/ps/products/168/ab168348/Images/ab168348-7-benefits-of-recombinant-antibodies.png)
Anti-Lumican antibody [EPR8898(2)] (ab168348)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (30)
ab168348 は 30 報の論文で使用されています。
- Chow SE et al. Nuclear p120 catenin is a component of the perichromosomal layer and coordinates sister chromatid segregation during mitosis in lung cancer cells. Cell Death Dis 13:526 (2022). PubMed: 35660718
- Wang K et al. Lumican is elevated in the lung in human and experimental acute respiratory distress syndrome and promotes early fibrotic responses to lung injury. J Transl Med 20:392 (2022). PubMed: 36059026
- Wu J et al. Effects of lumican expression on the apoptosis of scleral fibroblasts: In vivo and in vitro experiments. Exp Ther Med 21:495 (2021). PubMed: 33791004
- Kafarnik C et al. Canine Corneal Stromal Cells Have Multipotent Mesenchymal Stromal Cell Properties In Vitro. Stem Cells Dev 29:425-439 (2020). PubMed: 31973649
- Denes BJ et al. Core Matrisome Protein Signature During Periodontal Ligament Maturation From Pre-occlusal Eruption to Occlusal Function. Front Physiol 11:174 (2020). PubMed: 32194440