Anti-LTBP1 抗体 (ab78294)
Key features and details
- Rabbit polyclonal to LTBP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-LTBP1 antibody -
製品の詳細
Rabbit polyclonal to LTBP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Cow, Human
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免疫原
Synthetic peptide corresponding to Human LTBP1 aa 1350-1450 conjugated to keyhole limpet haemocyanin.
(Peptide available asab87128) -
ポジティブ･コントロール
- This antibody gave a positive signal in the following tissue lysates: Rat Liver; Mouse Pancreas; Mouse Spleen. ]# This antibody gave a positive result in IHC in the following FFPE tissue: Mouse spleen.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab78294の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 187 kDa (predicted molecular weight: 187 kDa).
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| IHC-P | (1) |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 187 kDa (predicted molecular weight: 187 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
May be involved in the assembly, secretion and targeting of TGFB1 to sites at which it is stored and/or activated. May play critical roles in controlling and directing the activity of TGFB1. May have a structural role in the extra cellular matrix (ECM). -
組織特異性
Isoform Long is found in fibroblasts. -
配列類似性
Belongs to the LTBP family.
Contains 18 EGF-like domains.
Contains 4 TB (TGF-beta binding) domains. -
ドメイン
Associates covalently with small latent TGF-beta complex via domain TB 3. -
翻訳後修飾
Contains hydroxylated asparagine residues.
Isoform Short N-terminus is blocked.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 4052 Human
- Entrez Gene: 268977 Mouse
- Entrez Gene: 59107 Rat
- Omim: 150390 Human
- SwissProt: Q14766 Human
- SwissProt: Q8CG19 Mouse
- SwissProt: Q00918 Rat
- Unigene: 619315 Human
see all -
別名
- 9430031G15Rik antibody
- 9830146M04 antibody
- Latent transforming growth factor beta binding protein 1 antibody
see all
画像
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Western blot - Anti-LTBP1 antibody (ab78294)All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lane 3 : Spleen (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 187 kDa
Observed band size: 187 kDa
Based on the immunogen sequence, this antibody is predicted to react with all three isoforms of LTBP1. The band detected here might correspond to the long isoform, which is 187 kDa and is found in fibroblasts. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTBP1 antibody (ab78294)IHC image of LTBP1 staining in Mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78294, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-LTBP1 antibody (ab78294)All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 187 kDa
Observed band size: 187 kDa
Additional bands at: 171 kDa (possible cleavage fragment), 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab78294 は 12 報の論文で使用されています。
- Gu H et al. Immune suppressive signaling regulated by latent transforming growth factor beta binding protein 1 promotes metastasis in cervical cancer. Braz J Med Biol Res 55:e12206 (2023). PubMed: 36629522
- Zhang RX et al. Intravitreal injection of fibrillin 2 (Fbn2) recombinant protein for therapy of retinopathy in a retina-specific Fbn2 knock-down mouse model. Sci Rep 13:6865 (2023). PubMed: 37100863
- Wu X et al. Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis. Eur Respir J 61:N/A (2023). PubMed: 36758986
- Bariani MV et al. TGFβ signaling links early life endocrine-disrupting chemicals exposure to suppression of nucleotide excision repair in rat myometrial stem cells. Cell Mol Life Sci 80:288 (2023). PubMed: 37689587
- Lian G et al. Bioinformatics analysis of the immune cell infiltration characteristics and correlation with crucial diagnostic markers in pulmonary arterial hypertension. BMC Pulm Med 23:300 (2023). PubMed: 37582718