Anti-LTB 抗体 (ab64835)
Key features and details
- Rabbit polyclonal to LTB
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-LTB antibody
LTB 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LTB -
由来種
Rabbit -
特異性
ab64835 detects endogenous levels of total Lymphotoxin Beta protein. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human LTB (C terminal).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64835の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
|
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| WB |
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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| 特記事項 |
|---|
|
ICC/IF
1/500 - 1/1000. |
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WB
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
ターゲット情報
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機能
Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface. Isoform 2 is probably non-functional. -
組織特異性
Spleen and thymus. -
配列類似性
Belongs to the tumor necrosis factor family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4050 Human
- Omim: 600978 Human
- SwissProt: Q06643 Human
- Unigene: 376208 Human
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別名
- LT beta antibody
- LT-beta antibody
- Ltb antibody
see all
画像
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Western blot - Anti-LTB antibody (ab64835)All lanes : Anti-LTB antibody (ab64835) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising peptide
Predicted band size: 25 kDa
Observed band size: 25 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug. -
Immunocytochemistry/ Immunofluorescence - Anti-LTB antibody (ab64835)ab64835 at 1/500 - 1/1000 dilution staining Lymphotoxin Beta in HeLa cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab64835 は 7 報の論文で使用されています。
- Morelli M et al. Experimental Methods for the Immunological Characterization of Paradoxical Psoriasis Reactions Induced by TNF-α Biologics. Methods Mol Biol 2248:155-165 (2021). PubMed: 33185874
- Fania L et al. Paradoxical psoriasis induced by TNF-a blockade shows immunological features typical of the early phase of psoriasis development. J Pathol Clin Res 6:55-68 (2020). PubMed: 31577850
- Francipane MG et al. Host Lymphotoxin-ß Receptor Signaling Is Crucial for Angiogenesis of Metanephric Tissue Transplanted into Lymphoid Sites. Am J Pathol 190:252-269 (2020). PubMed: 31585070
- Cortes-Selva D et al. IL-4 promotes stromal cell expansion and is critical for development of a type-2, but not a type 1 immune response. Eur J Immunol 49:428-442 (2019). PubMed: 30575951
- Yim RL et al. Methylation of miR-155-3p in mantle cell lymphoma and other non-Hodgkin's lymphomas. Oncotarget 5:9770-82 (2014). Flow Cyt . PubMed: 25211095
- Simonin Y et al. Lymphotoxin signaling is initiated by the viral polymerase in HCV-linked tumorigenesis. PLoS Pathog 9:e1003234 (2013). WB, IHC-P ; Mouse . PubMed: 23555249
- Watanabe T et al. Higher-order chromatin regulation and differential gene expression in the human tumor necrosis factor/lymphotoxin locus in hepatocellular carcinoma cells. Mol Cell Biol 32:1529-41 (2012). IHC-P ; Human . PubMed: 22354988