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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-LRRK2 抗体 [MJFF2 (c41-2)] - BSA and Azide free (ab172378)

Submit a review Submit a question References (2)
Western blot - Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)
  • Immunoprecipitation - Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)
  • Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MJFF2 (c41-2)] to LRRK2 - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

HRP Unconjugated

製品の概要

  • 製品名

    Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free
    LRRK2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [MJFF2 (c41-2)] to LRRK2 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Recombinant fragment within Human LRRK2 aa 950 to the C-terminus. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • WB: A549 and MEF cell lysates; HEK293 cells transfected with LRRK2 cell lysate. IP: Mouse cerebral cortex, A549 .

  • 特記事項

    ab172378 is the carrier-free version of ab133474.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This antibody was developed with support from The Michael J. Fox Foundation.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab172378の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 286 kDa.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 286 kDa.
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 機能

    Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease.

  • 組織特異性

    Expressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.

  • 関連疾患

    Parkinson disease 8

  • 配列類似性

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 12 LRR (leucine-rich) repeats.
    Contains 1 protein kinase domain.
    Contains 1 Roc domain.
    Contains 7 WD repeats.

  • ドメイン

    The seven-bladed WD repeat region is critical for synaptic vesicle trafficking and mediates interaction with multiple vesicle-associated presynaptic proteins.
    The Roc domain mediates homodimerization and regulates kinase activity.

  • 翻訳後修飾

    Autophosphorylated.

  • 細胞内局在

    Membrane. Cytoplasm. Perikaryon. Mitochondrion. Golgi apparatus. Cell projection, axon. Cell projection, dendrite. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Endosome. Lysosome. Mitochondrion outer membrane. Mitochondrion inner membrane. Mitochondrion matrix. Predominantly associated with intracytoplasmic vesicular and membranous structures (By similarity). Localized in the cytoplasm and associated with cellular membrane structures. Predominantly associated with the mitochondrial outer membrane of the mitochondria. Colocalized with RAB29 along tubular structures emerging from Golgi apparatus. Localizes in intracytoplasmic punctate structures of neuronal perikarya and dendritic and axonal processes.
  • Information by UniProt

  • 参照データベース

    • 別名

      • augmented in rheumatoid arthritis 17 antibody
      • AURA17 antibody
      • Dardarin antibody
      • Leucine rich repeat kinase 2 antibody
      • leucine rich repeat serine threonine protein kinase 2 antibody
      • Leucine-rich repeat serine/threonine-protein kinase 2 antibody
      • LRRK 2 antibody
      • LRRK2 antibody
      • LRRK2_HUMAN antibody
      • PARK 8 antibody
      • PARK8 antibody
      • RIPK7 antibody
      • ROCO 2 antibody
      • ROCO2 antibody
      see all

    画像

    • Western blot - Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)
      Western blot - Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)

      This WB data was generated using the same anti-LRRK2 antibody clone, MJFF2 (c41-2), in a different buffer formulation (cat# ab133474).

      Lane 1: Wild type A549 whole cell lysate (20 µg)
      Lane 2: Wild type MEF whole cell lysate (20 µg)
      Lane 3: LRRK2 knockout A549 whole cell lysate (20 µg)
      Lane 4: LRRK2 knockout MEF whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab133474 observed at 286 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab133474 was shown to recognize LRRK2 in wild type A549 and MEF cells along with additional cross reative bands. Whilst signal was not seen in LRRK2 knockout cells. Wild-type and LRRK2 knockout samples were subjected to SDS-PAGE. Ab133474 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

      Wild-type and LRRK2 knockout MEF and A549 cells were provide as a generous gift from Professor Dario Alessi, MRC Protein Phosphorylation and Ubiquitination Unit (University of Dundee).

    • Immunoprecipitation - Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)
      Immunoprecipitation - Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)

      This data was developed using ab133474, the same antibody clone in a different buffer formulation.

      LRRK2 was immunoprecipitated from 0.35 mg A549 (Human lung carcinoma epithelial cell) whole cell lysate 10 µg with ab133474 at 1/60 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysate 10 µg

      Lane 2: abab133474 IP in A549 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133474 in A549 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Fresh lysate should be used to minimize protein degradation.

    • Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)
      Anti-LRRK2 antibody [MJFF2 (c41-2)] - BSA and Azide free (ab172378)

    参考文献 (2)

    ab172378 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab172378 は 2 報の論文で使用されています。

    • Obergasteiger J  et al. The small GTPase Rit2 modulates LRRK2 kinase activity, is required for lysosomal function and protects against alpha-synuclein neuropathology. NPJ Parkinsons Dis 9:44 (2023). PubMed: 36973269
    • Obergasteiger J  et al. Kinase inhibition of G2019S-LRRK2 enhances autolysosome formation and function to reduce endogenous alpha-synuclein intracellular inclusions. Cell Death Discov 6:45 (2020). PubMed: 32550012

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