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AB320724

Anti-LRRC15 抗体 [EPR28303-108] - BSA and Azide free

Anti-LRRC15 antibody [EPR28303-108] - BSA and Azide free

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Rabbit Recombinant Monoclonal LRRC15 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human, Mouse samples.

別名を表示する

LIB, LRRC15, Leucine-rich repeat-containing protein 15, Leucine-rich repeat protein induced by beta-amyloid homolog, hLib

4 Images
Flow Cytometry - Anti-LRRC15 antibody [EPR28303-108] - BSA and Azide free (AB320724)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-LRRC15 antibody [EPR28303-108] - BSA and Azide free (AB320724)

This data was developed using ab320723, the same antibody clone in a different buffer formulation. Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / U-118 MG (human brain glioblastoma cell, Right) cells labelling LRRC15 with ab320723 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody. Low expression : MCF7 (PMID : 35260879). Gated on viable cells.

Flow Cytometry - Anti-LRRC15 antibody [EPR28303-108] - BSA and Azide free (AB320724)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-LRRC15 antibody [EPR28303-108] - BSA and Azide free (AB320724)

This data was developed using ab320723, the same antibody clone in a different buffer formulation. Flow cytometric analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell) treated with 20ng/ml TGF beta 1 for 48h (Green) /Untreated control (Magenta) cells labelling LRRC15 with ab320723 at 1/50 dilution (1ug) /Magenta and Green compared with a Rabbit monoclonal IgG (ab172730) (Black and Grey) isotype control. Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody. Gated on viable cells.

Flow Cytometry - Anti-LRRC15 antibody [EPR28303-108] - BSA and Azide free (AB320724)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-LRRC15 antibody [EPR28303-108] - BSA and Azide free (AB320724)

This data was developed using ab320723, the same antibody clone in a different buffer formulation. Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell, Left) / U-87 MG (human glioblastoma-astrocytoma epithelial cell, Right) cells labelling LRRC15 with ab320723 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody. Low expression : HeLa (PMID : 33960636). Gated on viable cells.

Flow Cytometry - Anti-LRRC15 antibody [EPR28303-108] - BSA and Azide free (AB320724)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-LRRC15 antibody [EPR28303-108] - BSA and Azide free (AB320724)

This data was developed using ab320723, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of MEF (mouse embryo fibroblast, Left) / C2C12 (mouse myoblast, Right) cells labelling LRRC15 with ab320723 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Negative control : MEF.
Gated on viable cells.

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR28303-108

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human, Mouse

アプリケーション

Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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製品の詳細

ab320724 is the carrier-free version of ab320723.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: 100% PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

(Microbial infection) Modulates the ability of SARS-CoV-2 to infect host cells through interaction with the spike protein (PubMed : 36228039, PubMed : 36735681, PubMed : 36757924). Does not act as a SARS-CoV-2 entry receptor but sequesters virions and antagonizes in trans SARS-CoV-2 infection of ACE2(+) cells when expressed on nearby cells (PubMed : 36228039, PubMed : 36757924).
See full target information LRRC15
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Abcam product promise

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