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リコンビナントRabMAb

Anti-LRPAP1 抗体 [EPR3327] (ab76500)

Submit a review Q&A (1)References (2)
Western blot - Anti-LRPAP1 antibody [EPR3327] (ab76500)
  • Western blot - Anti-LRPAP1 antibody [EPR3327] (ab76500)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)
  • Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500)
  • Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500)
  • Anti-LRPAP1 antibody [EPR3327] (ab76500)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3327] to LRPAP1
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-LRPAP1 antibody [EPR3327]
    LRPAP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3327] to LRPAP1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, IHC-P, WBmore details
    適用なし: Flow Cyt or ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human LRPAP1 aa 1-100. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • WB: Human fetal kidney, mouse kidney and rat kidney tissue lysate and 293T cell lysates. IHC-P: Human hepatocellular carcinoma and brain tissues. IP: Human fetal kidney tissue lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76500の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/50 - 1/200.
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
特記事項
IP
1/50 - 1/200.
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
追加情報
Is unsuitable for Flow Cyt or ICC/IF.

ターゲット情報

  • 機能

    Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.

  • 関連疾患

    Note=In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis.

  • 配列類似性

    Belongs to the alpha-2-MRAP family.

  • 翻訳後修飾

    N-glycosylated.

  • 細胞内局在

    Endoplasmic reticulum. Cytoplasm. Cell surface. Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 39 kDa receptor-associated protein antibody
    • A2MRAP antibody
    • A2RAP antibody
    • Alpha 2 macroglobulin receptor associated protein antibody
    • Alpha 2 MRAP antibody
    • Alpha-2-macroglobulin receptor-associated protein antibody
    • Alpha-2-MRAP antibody
    • AMRP_HUMAN antibody
    • HBP44 antibody
    • Lipoprotein receptor associated protein antibody
    • Low density lipoprotein receptor related protein associated protein 1 antibody
    • Low density lipoprotein receptor-related protein-associated protein 1 antibody
    • Low density lipoprotein related protein associated protein 1 alpha 2 macroglobulin receptor associated protein antibody
    • Low density lipoprotein related protein associated protein 1 antibody
    • low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1) antibody
    • Lrpap1 antibody
    • MGC138272 antibody
    • MRAP antibody
    • MYP23 antibody
    • RAP antibody
    see all

画像

  • Western blot - Anti-LRPAP1 antibody [EPR3327] (ab76500)
    Western blot - Anti-LRPAP1 antibody [EPR3327] (ab76500)
    All lanes : Anti-LRPAP1 antibody [EPR3327] (ab76500) at 1/6000 dilution (unpurified)

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Rat kidney tissue lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST

  • Western blot - Anti-LRPAP1 antibody [EPR3327] (ab76500)
    Western blot - Anti-LRPAP1 antibody [EPR3327] (ab76500)
    All lanes : Anti-LRPAP1 antibody [EPR3327] (ab76500) at 1/3000 dilution (purified)

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Rat kidney tissue lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling LRPAP1 with unpurified ab76500 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling LRPAP1 with purified ab76500 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling LRPAP1 with unpurified ab76500 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500)
    Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500)

    ab76500 (unpurified) at 1/300 immunoprecipitating LRPAP1 in human fetal kidney tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500)
    Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500)

    ab76500 (purified) at 1/150 immunoprecipitating LRPAP1 in human fetal kidney tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-LRPAP1 antibody [EPR3327] (ab76500)
    Anti-LRPAP1 antibody [EPR3327] (ab76500)

参考文献 (2)

ab76500 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab76500 は 2 報の論文で使用されています。

  • Li H  et al. Secreted LRPAP1 binds and triggers IFNAR1 degradation to facilitate virus evasion from cellular innate immunity. Signal Transduct Target Ther 8:374 (2023). PubMed: 37743411
  • Marakasova E  et al. Molecular chaperone RAP interacts with LRP1 in a dynamic bivalent mode and enhances folding of ligand-binding regions of other LDLR family receptors. J Biol Chem 297:100842 (2021). PubMed: 34058195

レビューと Q&A

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Question

will send his sequence
align with human
check which Ab might be best choice

human, WB

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Answer

Thank you for contacting us.

As we discussed over the phone, please send me your sequence so that I can find an antibody that works best for your purposes.

I look forward to hear back and help you further.

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