Anti-LRP4 抗体 (ab85697)
Key features and details
- Goat polyclonal to LRP4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-LRP4 antibody
LRP4 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to LRP4 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Synthetic peptide:
C-SNPSYRTSTQEVK
, corresponding to internal sequence amino acids 1765-1777 of Human LRP4 (NP_002325.2). -
ポジティブ･コントロール
- Human brain (cerebral cortex) lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab85697 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85697の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 216 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 216 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Potential cell surface endocytic receptor, which binds and internalizes extracellular ligands for degradation by lysosomes. Involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. -
組織特異性
Expressed in several regions of the brain. -
関連疾患
Defects in LRP4 are the cause of Cenani-Lenz syndactyly syndrome (CLSS) [MIM:212780]. It is a congenital malformation syndrome defined as complete and complex syndactyly of the hands combined with malformations of the forearm bones and similar manifestations in the lower limbs. -
配列類似性
Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 8 LDL-receptor class A domains.
Contains 20 LDL-receptor class B repeats. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 504317 Cow
- Entrez Gene: 4038 Human
- Entrez Gene: 228357 Mouse
- Entrez Gene: 83469 Rat
- Omim: 604270 Human
- SwissProt: O75096 Human
- SwissProt: Q8VI56 Mouse
- SwissProt: Q9Z319 Mouse
see all -
別名
- KIAA0816 antibody
- LDLR dan antibody
- Low density lipoprotein receptor related protein 4 antibody
see all
画像
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Western blot - Anti-LRP4 antibody (ab85697)Anti-LRP4 antibody (ab85697) at 0.1 µg/ml + Human cerebral cortex lysate (in RIPA buffer) at 35 µg
Predicted band size: 216 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab85697 は 3 報の論文で使用されています。
- Ohkawara B et al. CTGF/CCN2 facilitates LRP4-mediated formation of the embryonic neuromuscular junction. EMBO Rep 21:e48462 (2020). PubMed: 32558157
- Selcen D et al. Impaired Synaptic Development, Maintenance, and Neuromuscular Transmission in LRP4-Related Myasthenia. JAMA Neurol 72:889-96 (2015). PubMed: 26052878
- Ohkawara B et al. LRP4 third ß-propeller domain mutations cause novel congenital myasthenia by compromising agrin-mediated MuSK signaling in a position-specific manner. Hum Mol Genet N/A:N/A (2013). PubMed: 24234652