Anti-LRP1 抗体 (ab168454)
Key features and details
- Mouse polyclonal to LRP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LRP1 antibody
LRP1 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to LRP1 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Recombinant fragment corresponding to amino acids 1-292 of Human LRP1. (AAH45107.1)
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ポジティブ･コントロール
- Transfected 293T cell lysate; Human endometrium tissue; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168454の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa.
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| IHC-P |
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa. |
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IHC-P
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
Functions as a receptor for Pseudomonas aeruginosa exotoxin A. -
組織特異性
Most abundant in liver, brain and lung. -
配列類似性
Belongs to the LDLR family.
Contains 22 EGF-like domains.
Contains 31 LDL-receptor class A domains.
Contains 34 LDL-receptor class B repeats. -
翻訳後修飾
Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.
The N-terminus is blocked.
Phosphorylated on serine and threonine residues.
Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1. -
細胞内局在
Cytoplasm. Nucleus. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus and Cell membrane. Membrane, coated pit. - Information by UniProt
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参照データベース
- Entrez Gene: 4035 Human
- Entrez Gene: 16971 Mouse
- Omim: 107770 Human
- SwissProt: Q07954 Human
- SwissProt: Q91ZX7 Mouse
- Unigene: 162757 Human
- Unigene: 271854 Mouse
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別名
- A2MR antibody
- Alpha 2 macroglobulin receptor antibody
- alpha 2MR antibody
see all
画像
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Western blot - Anti-LRP1 antibody (ab168454)All lanes : Anti-LRP1 antibody (ab168454) at 1 µg/ml
Lane 1 : LRP1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-mouse HRP at 1/2500 dilution -
Immunocytochemistry/ Immunofluorescence - Anti-LRP1 antibody (ab168454)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LRP1 with ab168454 at 10µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP1 antibody (ab168454)Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human endometrium tissue labeling LRP1 with ab168454 at 3µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab168454 は 2 報の論文で使用されています。
- Zheng Y et al. Anti-PAI-1 Monoclonal Antibody Inhibits the Metastasis and Growth of Esophageal Squamous Cell Carcinoma. J Cancer 14:114-128 (2023). PubMed: 36605486
- Ma J et al. Neutrophil elastase-regulated macrophage sheddome/secretome and phagocytic failure. Am J Physiol Lung Cell Mol Physiol 321:L555-L565 (2021). PubMed: 34261337
