Anti-LRP1 抗体 [8G1] (ab20384)
Key features and details
- Mouse monoclonal [8G1] to LRP1
- Suitable for: ICC/IF, Electron Microscopy
- Reacts with: Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-LRP1 antibody [8G1]
LRP1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [8G1] to LRP1 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, Electron Microscopymore details -
種交差性
交差種: Rat, Human -
免疫原
Full length protein corresponding to LRP1. J Biol Chem 265:17401-4 (1990).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.6
Constituents: 0.82% Sodium phosphate, 0.58% Sodium chloride -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
8G1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab20384の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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Electron Microscopy |
Use at an assay dependent concentration. Use at dilutions of 1/400 or 1/600 for 20 mins.
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特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
Electron Microscopy
Use at an assay dependent concentration. Use at dilutions of 1/400 or 1/600 for 20 mins. |
ターゲット情報
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機能
Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
Functions as a receptor for Pseudomonas aeruginosa exotoxin A. -
組織特異性
Most abundant in liver, brain and lung. -
配列類似性
Belongs to the LDLR family.
Contains 22 EGF-like domains.
Contains 31 LDL-receptor class A domains.
Contains 34 LDL-receptor class B repeats. -
翻訳後修飾
Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.
The N-terminus is blocked.
Phosphorylated on serine and threonine residues.
Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1. -
細胞内局在
Cytoplasm. Nucleus. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus and Cell membrane. Membrane, coated pit. - Information by UniProt
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参照データベース
- Entrez Gene: 4035 Human
- Entrez Gene: 299858 Rat
- Omim: 107770 Human
- SwissProt: Q07954 Human
- Unigene: 162757 Human
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別名
- A2MR antibody
- Alpha 2 macroglobulin receptor antibody
- alpha 2MR antibody
see all
画像
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ICC/IF image of ab20384 stained Mcf7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20384, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Ab20384 staining primary rat hepatocytes cells by EM. The cells were fixed and prepared by Tokuyasu method. The cryosections of 70nm were placed on 100 mesh grids. All the following steps were done at room temperature. Blocking was carried out for 30 min with 1% fish skin gelatine (FSG). The tissue sections were placed on a drop of 5 ul of first Ab (anti-LRP 8G1) for 20 min at dilutions of 1:400 or 1:600 seemed to work the best. After incubation with the primary antibody, 3. 5 x 2 min wash was done with PBS and the sections were placed on a drop of 5 ul of secondary Ab (goat anti-mouse) for 20 min (dilution 1:150 5. 5 x 2 min wash with PBS). Samples were placed on a drop of 5 ul of PAG10 for 15 min (dilution 1:60 or 1:70) and then 5 x 2 min wash with PBS. 2 min on 1% glutaraldehyde for fixation and 4 x 2.5 min on H2O. afterthat 1 second + 1 second + 6 min on drops of Methyl Cellulose/Uranyl Acetate (9:1) on ice. The surplus of MC/UA was removed , the slides were dried and obser
データシートおよび資料
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Datasheet download
参考文献 (13)
ab20384 は 13 報の論文で使用されています。
- Remacle AG et al. Interaction of the cryptic fragment of myelin basic protein with mitochondrial voltage-dependent anion-selective channel-1 affects cell energy metabolism. Biochem J 475:2355-2376 (2018). PubMed: 29954845
- Chan CY et al. Clearance of matrix metalloproteinase-9 is dependent on low-density lipoprotein receptor-related protein-1 expression downregulated by microRNA-205 in human abdominal aortic aneurysm. J Vasc Surg 65:509-520 (2017). PubMed: 26781079
- Boyé K et al. The role of CXCR3/LRP1 cross-talk in the invasion of primary brain tumors. Nat Commun 8:1571 (2017). PubMed: 29146996
- Chanalaris A et al. Suramin Inhibits Osteoarthritic Cartilage Degradation by Increasing Extracellular Levels of Chondroprotective Tissue Inhibitor of Metalloproteinases 3. Mol Pharmacol 92:459-468 (2017). PubMed: 28798097
- Wilkinson DJ et al. Matriptase Induction of Metalloproteinase-Dependent Aggrecanolysis In Vitro and In Vivo: Promotion of Osteoarthritic Cartilage Damage by Multiple Mechanisms. Arthritis Rheumatol 69:1601-1611 (2017). PubMed: 28464560