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リコンビナントRabMAb

Anti-LRG1/LRG 抗体 [EPR12363] (ab170953)

Submit a review Q&A (1)References (2)
Immunoprecipitation - Anti-LRG1/LRG antibody [EPR12363] (ab170953)
  • Western blot - Anti-LRG1/LRG antibody [EPR12363] (ab170953)
  • Anti-LRG1/LRG antibody [EPR12363] (ab170953)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12363] to LRG1/LRG
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-LRG1/LRG antibody [EPR12363]
    LRG1/LRG 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12363] to LRG1/LRG

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: ICC/IF or IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human LRG1/LRG aa 1-100. The exact sequence is proprietary.
    Database link: P02750

  • ポジティブ･コントロール

    • WB: Human plasma.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab170953の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IP
1/10 - 1/100.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IP
1/10 - 1/100.
追加情報
Is unsuitable for ICC/IF or IHC-P.

ターゲット情報

  • 組織特異性

    Plasma.

  • 配列類似性

    Contains 8 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 1300008B03Rik antibody
      • 2310031E04Rik antibody
      • A2GL antibody
      • A2GL_HUMAN antibody
      • HMFT1766 antibody
      • Leucine rich alpha 2 glycoprotein antibody
      • Leucine-rich alpha-2-glycoprotein 1 antibody
      • Leucine-rich alpha-2-glycoprotein antibody
      • Leucine-rich alpha-2-glycoprotein precursor antibody
      • LRG antibody
      • LRG1 antibody
      see all

    画像

    • Immunoprecipitation - Anti-LRG1/LRG antibody [EPR12363] (ab170953)
      Immunoprecipitation - Anti-LRG1/LRG antibody [EPR12363] (ab170953)

      Western blot analysis of Immunoprecipitation pellet from either 1) Human plasma lysate, or 2) 1xPBS (negative control); showing LRG1/LRG, using ab170953 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Western blot - Anti-LRG1/LRG antibody [EPR12363] (ab170953)
      Western blot - Anti-LRG1/LRG antibody [EPR12363] (ab170953)
      Anti-LRG1/LRG antibody [EPR12363] (ab170953) at 1/1000 dilution + Human plasma at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 38 kDa

    • Anti-LRG1/LRG antibody [EPR12363] (ab170953)
      Anti-LRG1/LRG antibody [EPR12363] (ab170953)

    参考文献 (2)

    ab170953 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab170953 は 2 報の論文で使用されています。

    • Luan L  et al. Knockdown of lncRNA NEAT1 expression inhibits cell migration, invasion and EMT by regulating the miR-24-3p/LRG1 axis in retinoblastoma cells. Exp Ther Med 21:367 (2021). PubMed: 33732340
    • Druhan LJ  et al. Leucine Rich a-2 Glycoprotein: A Novel Neutrophil Granule Protein and Modulator of Myelopoiesis. PLoS One 12:e0170261 (2017). ELISA ; Human . PubMed: 28081565

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    What was the protocol for IP of human plasma? In particular, what volume of plasma was used, and how much antibody was added?

    Read More
    Answer

    For immunoprecipitation of LRG1 from plasma, we added 6ul of the antibody into 300ul of the plasma lysates (the protein concentration in the lysate is about 3.5mg/ml).

    Plasma lysate preparation
    1. Whole blood with anticoagulant. (Recommend using the fresh whole blood within 6 to 8 hours)
    2. Centrifuge at 5000g for 7min at 2 ˜ 6 ℃;
    3. First layer is the plasma. Gently separate plasma using siphon;
    4. Measure the protein concentration using BCA method.
    5. Adjust the protein concentration of the lysate to 3.5 mg/ml with IP lysis buffer.

    IP lysis buffer:
    20mM Tris-HCl (pH7.5)
    150mM NaCl
    1% Triton
    1mM Na2EDTA
    1mM EGTA
    2.5 mM sodium pyrophosphate
    1mM β-glycerophosphate
    1mM Na3VO4
    1ug/mL Leupeptin
    1mM PMSF

    Read More

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