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リコンビナントRabMAb

Anti-LRFN5 抗体 [EPR24417-5] (ab302510)

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Western blot - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
  • Western blot - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
  • Immunoprecipitation - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
  • Immunoprecipitation - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
  • Anti-LRFN5 antibody [EPR24417-5] (AB302510)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24417-5] to LRFN5
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-LRFN5 antibody [EPR24417-5]
    LRFN5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24417-5] to LRFN5

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WB, IPmore details
    適用なし: ICC/IF or IHC-Fr

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse hippocampus, brain , spinal cord, and heart, rat hippocampus, brain and heart, tissue lysates; IHC-P: Mouse and rat cerebrum FFPE tissue sections. IP: Mouse and rat brain tissue lysates.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR24417-5

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302510の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 80-100 kDa (predicted molecular weight: 79 kDa).
IP
1/30.
特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 80-100 kDa (predicted molecular weight: 79 kDa).
IP
1/30.
追加情報
Is unsuitable for ICC/IF or IHC-Fr.

ターゲット情報

  • 機能

    Cell adhesion molecule that mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Promotes neurite outgrowth in hippocampal neurons.

  • 配列類似性

    Belongs to the LRFN family.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.

  • ドメイン

    Lacks a cytoplasmic PDZ-binding motif, which has been implicated in function of related LRFN proteins.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C14orf146 antibody
      • DKFZp686G0210 antibody
      • Fibronectin type III immunoglobulin and leucine rich repeat domains 8 antibody
      • FIGLER8 antibody
      • FLJ30803 antibody
      • leucine rich repeat and fibronectin type III domain containing 5 antibody
      • Leucine-rich repeat and fibronectin type-III domain-containing protein 5 antibody
      • Lrfn5 antibody
      • LRFN5_HUMAN antibody
      • SALM5 antibody
      see all

    画像

    • Western blot - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
      Western blot - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
      All lanes : Anti-LRFN5 antibody [EPR24417-5] (ab302510) at 1/1000 dilution

      Lane 1 : Mouse hippocampus tissue lysate
      Lane 2 : Mouse heart tissue lysate
      Lane 3 : Rat hippocampus tissue lysate
      Lane 4 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 79 kDa
      Observed band size: 80-100 kDa why is the actual band size different from the predicted?


      Exposure time: 48 seconds


      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

      LRFN5 is glycosylated protein. The molecular weights are consistent with what has been described in the literature (PMID: 16828986, 27152329).

      Negative control: heart (PMID: 27152329, 27152329).

      Samples are non-boiled as boiling may cause protein aggregates.

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    • Western blot - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
      Western blot - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
      All lanes : Anti-LRFN5 antibody [EPR24417-5] (ab302510) at 1/1000 dilution

      Lane 1 : Rat brain tissue lysate
      Lane 2 : Mouse brain tissue lysate
      Lane 3 : Mouse spinal cord tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 79 kDa
      Observed band size: 80-100 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

      LRFN5 is glycosylated protein. The molecular weights are consistent with what has been described in the literature (PMID: 16828986, 27152329).

      Samples are non-boiled as boiling may cause protein aggregates.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRFN5 antibody [EPR24417-5] (AB302510)

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling LRFN5 with ab302510 at 1/100 dilution (5.16 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse cerebrum (PMID: 27152329). The section was incubated with ab302510 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRFN5 antibody [EPR24417-5] (AB302510)

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling LRFN5 with ab302510 at 1/100 dilution (5.16 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on rat cerebrum. The section was incubated with ab302510 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRFN5 antibody [EPR24417-5] (AB302510)

      Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle for labeling LRFN5 with ab302510 at 1/100 dilution (5.16 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining on mouse cardiac muscle (PMID: 27152329). The section was incubated with ab302510 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRFN5 antibody [EPR24417-5] (AB302510)

      Immunohistochemical analysis of paraffin-embedded rat cardiac muscle for labeling LRFN5 with ab302510 at 1/100 dilution (5.16 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining on rat cardiac muscle. The section was incubated with ab302510 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunoprecipitation - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
      Immunoprecipitation - Anti-LRFN5 antibody [EPR24417-5] (AB302510)

      LRFN5 was immunoprecipitated from 10μg of mouse brain tissue lysate with ab302510 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab302510 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used as secondary antibody at 1/5000 dilution.

      Lane 1: Mouse brain tissue lysate 10μg (Input).

      Lane 2: ab302510 IP in mouse brain tissue lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302510 in mouse brain tissue lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 67 seconds.

    • Immunoprecipitation - Anti-LRFN5 antibody [EPR24417-5] (AB302510)
      Immunoprecipitation - Anti-LRFN5 antibody [EPR24417-5] (AB302510)

      LRFN5 was immunoprecipitated from 10μg of rat brain tissue lysate with ab302510 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab302510 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used as secondary antibody at 1/5000 dilution.

      Lane 1: Rat brain tissue lysate 10μg (Input).

      Lane 2: ab302510 IP in rat brain tissue lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302510 in rat brain tissue lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 67 seconds.

    • Anti-LRFN5 antibody [EPR24417-5] (AB302510)
      Anti-LRFN5 antibody [EPR24417-5] (AB302510)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302510 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302510 は論文での使用が確認できていません。

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