Anti-LRFN2 抗体 (ab106366)
Key features and details
- Rabbit polyclonal to LRFN2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-LRFN2 antibody -
製品の詳細
Rabbit polyclonal to LRFN2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human LRFN2 (C terminal). 14 amino acid peptide
Database link: Q9ULH4 -
ポジティブ･コントロール
- Rat brain tissue lysate, Mouse brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106366の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 85 kDa.
|
| 特記事項 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
|
IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 85 kDa. |
ターゲット情報
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機能
Promotes neurite outgrowth in hippocampal neurons. Enhances the cell surface expression of 2 NMDA receptor subunits GRIN1 and GRIN2A. May play a role in redistributing DLG4 to the cell periphery. -
配列類似性
Belongs to the LRFN family.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 7 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
ドメイン
The PDZ-binding motif is required for cell surface expression, neurite outgrowth promotion (By similarity). This motif is also involved in DLG1-, DLG3- and DLG4-binding. -
翻訳後修飾
Glycosylated. -
細胞内局在
Membrane. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 70530 Mouse
- Entrez Gene: 316205 Rat
- SwissProt: Q80TG9 Mouse
- SwissProt: Q460M5 Rat
- Unigene: 133607 Mouse
- Unigene: 47365 Rat
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別名
- fibronectin type III, immunoglobulin and leucine rich repeat domains 2 antibody
- FIGLER2 antibody
- KIAA1246 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-LRFN2 antibody (ab106366)
Immunofluorescence of LRFN2 in mouse brain tissue with ab106366 at 20 ug/mL.
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Western blot - Anti-LRFN2 antibody (ab106366)Lane 1 : Anti-LRFN2 antibody (ab106366) at 1 µg/ml
Lane 2 : Anti-LRFN2 antibody (ab106366) at 2 µg/ml
All lanes : Rat brain tissue lysate
Lysates/proteins at 15 µg/ml per lane.
Predicted band size: 85 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRFN2 antibody (ab106366)ab106366, at 2.5 ug/ml, staining LRFN2 in Mouse brain tissue by immunohistochemistry. -
Western blot - Anti-LRFN2 antibody (ab106366)Anti-LRFN2 antibody (ab106366) at 1 µg/ml + Mouse brain tissue lysate
Predicted band size: 85 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab106366 は 1 報の論文で使用されています。
- Kaur H et al. Contemporary Antiretroviral Therapy Dysregulates Iron Transport and Augments Mitochondrial Dysfunction in HIV-Infected Human Microglia and Neural-Lineage Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37569616