Anti-LOXL2 抗体 [EPR12733] - C-terminal (ab179810)
![Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810)](https://www.abcam.co.jp/ps/products/179/ab179810/Images/ab179810-384511-anti-loxl2-antibody-epr12733-c-terminal-western-blot-hela.jpg "Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12733] to LOXL2 - C-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-LOXL2 antibody [EPR12733] - C-terminal
LOXL2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12733] to LOXL2 - C-terminal -
由来種
Rabbit -
特異性
Our lab has been unable to use this product successfully in IHC-P and so we cannot guarantee that it will work in this application. However, some customers have been successful with this antibody in IHC-P. -
アプリケーション
適用あり: WBmore details
適用なし: IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human LOXL2 aa 750 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q9Y4K0 -
ポジティブ･コントロール
- WB: MDA-MB-435, A431, U87-MG, and HeLa cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12733 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab179810の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/200 - 1/1000. Predicted molecular weight: 87 kDa.
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| 特記事項 |
|---|
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WB
1/200 - 1/1000. Predicted molecular weight: 87 kDa. |
ターゲット情報
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組織特異性
Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate. -
配列類似性
Belongs to the lysyl oxidase family.
Contains 4 SRCR domains. -
翻訳後修飾
The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
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参照データベース
- Entrez Gene: 4017 Human
- Entrez Gene: 94352 Mouse
- Entrez Gene: 290350 Rat
- Omim: 606663 Human
- SwissProt: Q9Y4K0 Human
- SwissProt: P58022 Mouse
- SwissProt: B5DF27 Rat
- Unigene: 626637 Human
see all -
別名
- LOR 2 antibody
- LOR2 antibody
- LOX L2 antibody
see all
画像
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Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810)All lanes : Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : LOXL2 knockout HeLa cell lysate
Lane 3 : HeLa treated with 0.5nM CoCl2 for 6 hours whole cell lysate
Lane 4 : Untreated HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab179810 observed at 105 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab179810 Anti-LOXL2 antibody [EPR12733] - C-terminal was shown to specifically react with LOXL2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264807 (knockout cell lysate ab257166) was used. Wild-type and LOXL2 knockout samples were subjected to SDS-PAGE. ab179810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810)](https://www.abcam.co.jp/ps/products/179/ab179810/Images/ab179810-384459-anti-loxl2-antibody-epr12733-c-terminal-western-blot-hela-2.jpg)
Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810)All lanes : Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : LOXL2 knockout HeLa cell lysate
Lane 3 : HeLa treated with 0.5nM CoCl2 for 6 hours whole cell lysate
Lane 4 : Untreated HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab179810 observed at 105 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab179810 Anti-LOXL2 antibody [EPR12733] - C-terminal was shown to specifically react with LOXL2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261804 (knockout cell lysate ab257168) was used. Wild-type and LOXL2 knockout samples were subjected to SDS-PAGE. ab179810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810)](https://www.abcam.co.jp/ps/products/179/ab179810/Images/ab179810-292543-anti-loxl2-antibody-epr12733-c-terminal-western-blot.jpg)
Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810)Lanes 1-2 & 4 : Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810) at 1/1000 dilution
Lane 3 : Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810) at 1/200 dilution
Lane 1 : MCF-7 (Human breast carcinoma) whole cell lysate
Lane 2 : MDA-MB-435 (Human ductal carcinoma) whole cell lysate
Lane 3 : A431 (Human epidermoid carcinoma) whole cell lysate
Lane 4 : U87-MG (Human glioblastoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?Exposure time:
Lane 1-3: 3min
Lane 4: 1minBlocking/Diluting buffer and concentration 5% NFDM /TBST
MCF-7 lack LOXL2 expression (PMID: 19330836, PMID:12154058 and PMID: 27655685)
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![Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810)](https://www.abcam.co.jp/ps/products/179/ab179810/Images/ab179810-9-benefits-of-recombinant-antibodies.png)
Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab179810 は 14 報の論文で使用されています。
- Li N et al. Inhibitory effects of LOXL2 knockdown on cellular functions of liver cancer stem cells. Transl Cancer Res 11:2013-2025 (2022). PubMed: 35966289
- Cui Y et al. A novel epithelial-mesenchymal transition (EMT)-related gene signature of predictive value for the survival outcomes in lung adenocarcinoma. Front Oncol 12:974614 (2022). PubMed: 36185284
- Wen L et al. Phenylethanol glycosides from Herba Cistanche improve the hypoxic tumor microenvironment and enhance the effects of oxaliplatin via the HIF-1a signaling pathway. Mol Med Rep 24:N/A (2021). PubMed: 34013363
- Wang H et al. An in situ activity assay for lysyl oxidases. Commun Biol 4:840 (2021). PubMed: 34226627
- Klepfish M et al. LOXL2 Inhibition Paves the Way for Macrophage-Mediated Collagen Degradation in Liver Fibrosis. Front Immunol 11:480 (2020). PubMed: 32296422