Anti-LOX 抗体 (ab31238)
Key features and details
- Rabbit polyclonal to LOX
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LOX antibody
LOX 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LOX -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Dog
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免疫原
Synthetic peptide corresponding to Human LOX aa 400 to the C-terminus (C terminal).
(Peptide available asab28612) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31238の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (6) |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa).Can be blocked with Human LOX peptide (ab28612).
Abcam recommends using Milk as the blocking agent. |
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa).Can be blocked with Human LOX peptide (ab28612). Abcam recommends using Milk as the blocking agent. |
ターゲット情報
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機能
Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. -
組織特異性
Heart, placenta, skeletal muscle, kidney, lung and pancreas. -
関連疾患
Defects in LOX may be a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100]. -
配列類似性
Belongs to the lysyl oxidase family. -
翻訳後修飾
The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
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参照データベース
- Entrez Gene: 396474 Chicken
- Entrez Gene: 4015 Human
- Entrez Gene: 16948 Mouse
- Entrez Gene: 24914 Rat
- Omim: 153455 Human
- SwissProt: Q05063 Chicken
- SwissProt: P28300 Human
- SwissProt: P28301 Mouse
see all -
別名
- lox antibody
- LYOX antibody
- LYOX_HUMAN antibody
see all
画像
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Western blot - Anti-LOX antibody (ab31238)All lanes : Anti-LOX antibody (ab31238) at 1 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : Wild-type HeLa Treated DFO (0.5 mM, 24 h) cell lysate
Lane 3 : LOX knockout HeLa Vehicle Control DFO (0 mM, 24 h) cell lysate
Lane 4 : LOX knockout HeLa Treated DFO (0.5 mM, 24 h) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-LOX antibody staining at 1 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab31238 was shown to bind specifically to LOX. A band was observed at 50 kDa in wild-type HeLa cell lysates with no signal observed at this size in Lox knockout cell line ab261801 (knockout cell lysate ab256981). To generate this image, wild-type and Lox knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Western blot - Anti-LOX antibody (ab31238)All lanes : Anti-LOX antibody (ab31238) at 1 µg/ml
Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Additional bands at: 15 kDa, 36 kDa (possible mature (processed) protein), 47 kDa (possible immature (unprocessed)), 52 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab31238 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (77)
ab31238 は 77 報の論文で使用されています。
- D'Haese S et al. Moderate- and High-Intensity Endurance Training Alleviate Diabetes-Induced Cardiac Dysfunction in Rats. Nutrients 15:N/A (2023). PubMed: 37764732
- Yao Y et al. Pan-Lysyl Oxidase Inhibitor PXS-5505 Ameliorates Multiple-Organ Fibrosis by Inhibiting Collagen Crosslinks in Rodent Models of Systemic Sclerosis. Int J Mol Sci 23:N/A (2022). PubMed: 35628342
- Chaudhari N et al. Topical application of an irreversible small molecule inhibitor of lysyl oxidases ameliorates skin scarring and fibrosis. Nat Commun 13:5555 (2022). PubMed: 36138009
- Korntner SH et al. Craniofacial tendon development-Characterization of extracellular matrix morphology and spatiotemporal protein distribution. Front Cell Dev Biol 10:944126 (2022). PubMed: 36158210
- Adamo CS et al. EMILIN1 deficiency causes arterial tortuosity with osteopenia and connects impaired elastogenesis with defective collagen fibrillogenesis. Am J Hum Genet 109:2230-2252 (2022). WB, ICC/IF ; Mouse . PubMed: 36351433