AB190255

Anti-LNPEP 抗体 - N-terminal

Name: Anti-LNPEP 抗体 - N-terminal (ab190255) | Abcam
Brand: Abcam Limited
SKU: AB190255
Price: 73000 JPY
Availability: InStock

Anti-LNPEP antibody - N-terminal

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(1 Publication)

Rabbit Polyclonal LNPEP antibody. N-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LNPEP aa 1-50.

別名を表示する

OTASE, LNPEP, Leucyl-cystinyl aminopeptidase, Cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Insulin-responsive aminopeptidase, Oxytocinase, Placental leucine aminopeptidase, IRAP, OTase, P-LAP

2 Images

Supplier Data

Immunoprecipitation - Anti-LNPEP antibody - N-terminal (AB190255)

Detection of LNPEP by Western Blot of Immunoprecipitate.
ab190255 at 1 μg/ml labeling LNPEP in Jurkat whole cell lysate immunoprecipitated using ab190255 at 6 μg/reaction (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-LNPEP antibody - N-terminal (ab190255)

Predicted band size: 117 kDa

false

Supplier Data

Western blot - Anti-LNPEP antibody - N-terminal (AB190255)

All lanes:

Western blot - Anti-LNPEP antibody - N-terminal (ab190255) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 117 kDa

true

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human LNPEP aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9UIQ6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab190255 was affinity purified using an epitope specific to LNPEP immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

LNPEP also called leucyl/cystinyl aminopeptidase or insulin-regulated membrane aminopeptidase (IRAP) serves as a zinc-dependent aminopeptidase. This protein cleaves N-terminal amino acids from peptide substrates functioning inside various tissues like the heart brain and placenta. The mass of LNPEP is approximately 140 kDa. It associates with the endosome compartment in cells and resides at the plasma membrane linking to vesicular transport processes.

Biological function summary

LNPEP plays a role in peptide hormone regulation and recognition involving itself in glucose homeostasis and blood pressure regulation. It joins part of complexes involved in peptide recycling and processing within endocytic vesicles. This protein regulates the degradation of peptide hormones like oxytocin and vasopressin indicating its function extends to neuropeptide modulation.

Pathways

The activity of LNPEP spans significant biological pathways such as the renin-angiotensin system where it modulates the levels of angiotensin IV impacting cardiovascular functions and influencing blood pressure regulation. It closely relates to proteins like insulin-responsive aminopeptidase and angiotensin IV receptor enhancing its regulatory role within these pathways.

LNPEP significantly links to hypertension and diabetes. The modulation of angiotensin IV by LNPEP directly impacts blood pressure associating its activity with the development and management of hypertension. Additionally LNPEP's impact on glucose uptake and insulin action connects it with diabetic conditions. It exhibits interactions with proteins such as the glucose transporter type 4 (GLUT4) in the context of diabetes highlighting its role in metabolic regulation.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain.

See full target information LNPEP

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:199 PubMed40399808

2025

Metformin reverses 5-FU resistance induced by radiotherapy through mediating folate metabolism in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxuan Wang,Yanyan Lin,Qianqian Zhao,Huanliang Chen,Shisuo Du,Zhaochong Zeng

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LNPEP 抗体 - N-terminal

Anti-LNPEP antibody - N-terminal

Immunoprecipitation - Anti-LNPEP antibody - N-terminal (AB190255)

Western blot - Anti-LNPEP antibody - N-terminal (AB190255)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise