Anti-LMX1A 抗体 - C-terminal

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-LMX1A antibody - C-terminal (AB139726)

Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed HeLa cells labeling LMX1A with ab139726 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

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• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody - C-terminal (AB139726)

Immunohistochemical analysis of paraffin-embedded human brain meningioma tissue labeling LMX1A with ab139726 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody - C-terminal (AB139726)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling LMX1A with ab139726 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A antibody - C-terminal (AB139726)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling LMX1A with ab139726 at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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• ICC/IF

AbReview48407****

Immunocytochemistry/ Immunofluorescence - Anti-LMX1A antibody - C-terminal (AB139726)

ab139726 staining LMX1A (red) in mouse neural stem cells by ICC/IF (Immunocytochemistry/immunofluorescence).

Cells were fixed with formaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in PBS + 3% BSA + 0.3% Triton X-100) for 16 hours at 4°C. An Alexa-Fluor^® 568-conjugated donkey anti-rabbit IgG (H+L) monoclonal was used as the secondary antibody.

Blue - DAPI, Green - Nestin.

This image is courtesy of an Abreview submitted by Sébastien Brot

• WB

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Western blot - Anti-LMX1A antibody - C-terminal (AB139726)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-LMX1A antibody - C-terminal (ab139726) at 1 µg/mL

All lanes:

Human skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

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• WB

Supplier Data

Western blot - Anti-LMX1A antibody - C-terminal (AB139726)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-LMX1A antibody - C-terminal (ab139726) at 1 µg/mL

All lanes:

Rat skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

false

• WB

Supplier Data

Western blot - Anti-LMX1A antibody - C-terminal (AB139726)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-LMX1A antibody - C-terminal (ab139726) at 1 µg/mL

All lanes:

Mouse skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

false