Anti-LMAN1 抗体 [OTI1B8] (ab118407)
![Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)](https://www.abcam.co.jp/ps/products/118/ab118407/Images/ab118407-412977-anti-lman1-antibody-oti1b8-western-blot-hek293t-004104-wt-hek293t-lman1-knockout-hela-daudi.jpg "Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)")
Key features and details
- Mouse monoclonal [OTI1B8] to LMAN1
- Suitable for: WB
- Knockout validated
- Reacts with: Rat, Dog, Human, African green monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-LMAN1 antibody [OTI1B8]
LMAN1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [OTI1B8] to LMAN1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat, Dog, Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human LMAN1. Produced in HEK-293T cells (NP_005561).
Database link: P49257 -
ポジティブ･コントロール
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY LMAN1 cDNA; HEK-293T, Daudi, A549, COS-7, HeLa, HepG2, HT-29, Jurkat, MCF7, MDCK and PC-12 cell extracts.
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特記事項
Clone OTI1B8 (formerly 1B8).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Purified from cell culture supernatant by affinity chromatography.. -
ポリ/モノ
モノクローナル -
クローン名
OTI1B8 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118407の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/200 - 1/500. Predicted molecular weight: 58 kDa.
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| 特記事項 |
|---|
|
WB
1/200 - 1/500. Predicted molecular weight: 58 kDa. |
ターゲット情報
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機能
Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. -
組織特異性
Ubiquitous. -
関連疾患
Defects in LMAN1 are THE cause of factor V and factor VIII combined deficiency type 1 (F5F8D1) [MIM:227300]; also known as multiple coagulation factor deficiency I (MCFD1). F5F8D1 is an autosomal recessive blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal. -
配列類似性
Contains 1 L-type lectin-like domain. -
翻訳後修飾
The N-terminal may be partly blocked. -
細胞内局在
Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3998 Human
- Entrez Gene: 116666 Rat
- Omim: 601567 Human
- SwissProt: P49257 Human
- SwissProt: Q62902 Rat
- Unigene: 465295 Human
- Unigene: 25734 Rat
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別名
- Endoplasmic reticulum golgi intermediate compartment protein 53 antibody
- ER-Golgi intermediate compartment 53 kDa protein antibody
- ERGIC-53 antibody
see all
画像
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Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)All lanes : Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/200 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : LMAN1 knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab118407 observed at 55 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab118407 was shown to react with LMAN1 in wild-type HEK-293T cells in western blot with loss of signal observed in LMAN1 knockout cell line ab266248 (LMAN1 knockout cell lysate ab257505). Wild-type and LMAN1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab118407 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 200 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)](https://www.abcam.co.jp/ps/products/118/ab118407/Images/LMAN1-Primary-antibodies-ab118407-1.jpg)
Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)All lanes : Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/200 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY LMAN1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 58 kDa -
![Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)](https://www.abcam.co.jp/ps/products/118/ab118407/Images/LMAN1-Primary-antibodies-ab118407-2.jpg)
Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)All lanes : Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/200 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT29 (human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (canine kidney cell line) cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 58 kDa -
![Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)](https://www.abcam.co.jp/ps/products/118/ab118407/Images/ab118407-314448-ab118407WB3.jpg)
Western blot - Anti-LMAN1 antibody [OTI1B8] (ab118407)Lanes 1-2 : Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/1000 dilution
Lanes 3-4 : anti-HSP90AB1
Lanes 1 & 3 : Wild-type HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lanes 2 & 4 : LMAN1-knockout HEK-293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 58 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab118407 は 1 報の論文で使用されています。
- Si F et al. Porcine Epidemic Diarrhea Virus ORF3 Protein Is Transported through the Exocytic Pathway. J Virol 94:N/A (2020). PubMed: 32554695