Anti-LKB1 (phospho S428) 抗体 (ab63473)
Key features and details
- Rabbit polyclonal to LKB1 (phospho S428)
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-LKB1 (phospho S428) antibody
LKB1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LKB1 (phospho S428) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Human LKB1 (phospho S428).
Database link: Q15831 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab63473 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63473の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
|
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| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 48 kDa.
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| 特記事項 |
|---|
|
ICC/IF
1/500 - 1/1000. |
|
WB
1/500 - 1/1000. Predicted molecular weight: 48 kDa. |
ターゲット情報
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機能
Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor. -
組織特異性
Ubiquitously expressed. Strongest expression in testis and fetal liver. -
関連疾患
Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated by a cAMP-dependent protein kinase. -
細胞内局在
Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2. - Information by UniProt
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参照データベース
- Entrez Gene: 20869 Mouse
- SwissProt: Q9WTK7 Mouse
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別名
- hLKB1 antibody
- Liver kinase B1 antibody
- LKB1 antibody
see all
画像
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Western blot - Anti-LKB1 (phospho S428) antibody (ab63473)All lanes : Anti-LKB1 (phospho S428) antibody (ab63473) at 1/500 dilution
Lane 1 : mouse brain extracts
Lane 2 : mouse brain extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-LKB1 (phospho S428) antibody (ab63473)ab63473 at 1/500 dilution staining LKB1 in NIH3T3 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab63473 は 10 報の論文で使用されています。
- Gao J et al. Wnt family member 1 (Wnt1) overexpression-induced M2 polarization of microglia alleviates inflammation-sensitized neonatal brain injuries. Bioengineered 13:12409-12420 (2022). PubMed: 35603707
- Zhu C et al. lncRNA NBR2 attenuates angiotensin II-induced myocardial hypertrophy through repressing ER stress via activating LKB1/AMPK/Sirt1 pathway. Bioengineered 13:13667-13679 (2022). PubMed: 35703318
- Zhang J et al. PSPH induces cell autophagy and promotes cell proliferation and invasion in the hepatocellular carcinoma cell line Huh7 via the AMPK/mTOR/ULK1 signaling pathway. Cell Biol Int 45:305-319 (2021). PubMed: 33079432
- Wu J et al. Ferulic Acid Ameliorates Hepatic Inflammation and Fibrotic Liver Injury by Inhibiting PTP1B Activity and Subsequent Promoting AMPK Phosphorylation. Front Pharmacol 12:754976 (2021). PubMed: 34566665
- Kinsey CG et al. Protective autophagy elicited by RAF?MEK?ERK inhibition suggests a treatment strategy for RAS-driven cancers. Nat Med 25:620-627 (2019). PubMed: 30833748
- Hong B et al. Activation of the LKB1-SIK1 signaling pathway inhibits the TGF-ß-mediated epithelial-mesenchymal transition and apoptosis resistance of ovarian carcinoma cells. Mol Med Rep 17:2837-2844 (2018). PubMed: 29257268
- Ding Z et al. PCSK9 expression in the ischaemic heart and its relationship to infarct size, cardiac function, and development of autophagy. Cardiovasc Res 114:1738-1751 (2018). PubMed: 29800228
- Sueda T et al. BRAF V600E inhibition stimulates AMP-activated protein kinase-mediated autophagy in colorectal cancer cells. Sci Rep 6:18949 (2016). PubMed: 26750638
- Yang SH et al. Lovastatin overcomes gefitinib resistance through TNF-a signaling in human cholangiocarcinomas with different LKB1 statuses in vitro and in vivo. Oncotarget 6:23857-73 (2015). WB, IHC . PubMed: 26160843
- Konno H et al. Cyclic Dinucleotides Trigger ULK1 (ATG1) Phosphorylation of STING to Prevent Sustained Innate Immune Signaling. Cell 155:688-98 (2013). PubMed: 24119841