AB21356

Anti-Lipoprotein lipase 抗体 [LPL.A4]

Name: Anti-Lipoprotein lipase 抗体 [LPL.A4] (ab21356) | Abcam
Brand: Abcam Limited
SKU: AB21356
Rating: 4 (8 reviews)

Anti-Lipoprotein lipase antibody [LPL.A4]

(8 Reviews)

(64 Publications)

Mouse Monoclonal Lipoprotein lipase antibody. Suitable for Flow Cyt, WB and reacts with Human, Cow samples. Cited in 64 publications. Immunogen corresponding to Full Length Protein corresponding to Human LPL.

別名を表示する

LIPD, LPL, Lipoprotein lipase, Phospholipase A1

2 Images

Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

Flow Cyt

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Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

Overlay histogram showing HeLa cells stained with ab21356 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab21356, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

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Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

All lanes:

Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356) at 1/1000 dilution

Lane 1:

Human Lipoprotein lipase. at 10 µg

Lane 2:

Bovine Lipoprotein lipase at 10 µg

Predicted band size: 53 kDa

Observed band size: 55 kDa,60 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

LPL.A4

アイソタイプ

IgG1

キャリアフリー

交差種

Cow, Human

アプリケーション

Flow Cyt, WB

applications

免疫原

Full Length Protein corresponding to Human LPL. The exact immunogen used to generate this antibody is proprietary information.

P06858

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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製品の詳細

This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

精製に関する特記事項

Protein A affinity chromatography

バッファー組成

pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed : 11342582, PubMed : 27578112, PubMed : 8675619). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed : 12032167, PubMed : 7592706). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed : 24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed : 11342582, PubMed : 27811232).

See full target information LPL

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文献 (64)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400064 PubMed38981007

2024

Microglia Gravitate toward Amyloid Plaques Surrounded by Externalized Phosphatidylserine via TREM2.

Applications

Unspecified application

Species

Unspecified reactive species

Jong-Chan Park,Jong Won Han,Woochan Lee,Jieun Kim,Sang-Eun Lee,Dongjoon Lee,Hayoung Choi,Jihui Han,You Jung Kang,Yen N Diep,Hansang Cho,Rian Kang,Won Jong Yu,Jean Lee,Murim Choi,Sun-Wha Im,Jong-Il Kim,Inhee Mook-Jung

Immunology 170:28-46 PubMed37094816

2023

Deficiency of angiopoietin-like 4 enhances CD8 T cell bioactivity via metabolic reprogramming for impairing tumour progression.

Applications

Unspecified application

Species

Unspecified reactive species

Shizhen Ding,Zhijie Lin,Xiaoyuan Zhang,Xiaoqing Jia,Hualing Li,Yi Fu,Xuefeng Wang,Guoqiang Zhu,Guotao Lu,Weiming Xiao,Weijuan Gong

Frontiers in pharmacology 14:1148737 PubMed37077819

2023

Geniposide plus chlorogenic acid reverses non-alcoholic steatohepatitis regulation of gut microbiota and bile acid signaling in a mouse model .

Applications

Unspecified application

Species

Unspecified reactive species

Hongshan Li,Yingfei Xi,Xin Xin,Qin Feng,Yiyang Hu

Alzheimer's research & therapy 15:53 PubMed36918976

2023

Attenuation of Alzheimer's brain pathology in 5XFAD mice by PTH, a peptide of parathyroid hormone.

Applications

Unspecified application

Species

Unspecified reactive species

Li Chen,Lei Xiong,Lingling Yao,Jinxiu Pan,Emily Arzola,Xiaojuan Zhu,Lin Mei,Wen-Cheng Xiong

Molecular neurodegeneration 17:83 PubMed36536457

2022

Amyloid-beta and tau pathologies act synergistically to induce novel disease stage-specific microglia subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Won Kim,Kevin J Tu,Alice Wei,Ashley J Lau,Anabel Gonzalez-Gil,Tianyu Cao,Kerstin Braunstein,Jonathan P Ling,Juan C Troncoso,Philip C Wong,Seth Blackshaw,Ronald L Schnaar,Tong Li

Human molecular genetics 32:1466-1482 PubMed36519761

2022

Parkin regulates neuronal lipid homeostasis through SREBP2-lipoprotein lipase pathway-implications for Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Willcyn Tang,John Thundyil,Grace Gui Yin Lim,Teddy J W Tng,Sean Qing Zhang Yeow,Aditya Nair,Chou Chai,Tso-Pang Yao,Kah-Leong Lim

Frontiers in nutrition 9:914079 PubMed36091227

2022

Gypenosides ameliorate high-fat diet-induced non-alcoholic steatohepatitis farnesoid X receptor activation.

Applications

Unspecified application

Species

Unspecified reactive species

Hongshan Li,Yingfei Xi,Hongliang Liu,Xin Xin

Frontiers in pharmacology 13:848957 PubMed35652039

2022

Astilbin Activates the Reactive Oxidative Species/PPARγ Pathway to Suppress Effector CD4 T Cell Activities Direct Binding With Cytochrome P450 1B1.

Applications

Unspecified application

Species

Unspecified reactive species

Shizhen Ding,Guotao Lu,Biying Wang,Jie Xiang,Chunxia Hu,Zhijie Lin,Yanbing Ding,Weiming Xiao,Weijuan Gong

Journal of neuroinflammation 19:61 PubMed35236374

2022

Microglial VPS35 deficiency impairs Aβ phagocytosis and Aβ-induced disease-associated microglia, and enhances Aβ associated pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Ren,Lingling Yao,YongGang Wang,Lin Mei,Wen-Cheng Xiong

Nature communications 13:945 PubMed35177618

2022

Stroke induces disease-specific myeloid cells in the brain parenchyma and pia.

Applications

Unspecified application

Species

Unspecified reactive species

Carolin Beuker,David Schafflick,Jan-Kolja Strecker,Michael Heming,Xiaolin Li,Jolien Wolbert,Antje Schmidt-Pogoda,Christian Thomas,Tanja Kuhlmann,Irene Aranda-Pardos,Noelia A-Gonzalez,Praveen Ashok Kumar,Yves Werner,Ertugrul Kilic,Dirk M Hermann,Heinz Wiendl,Ralf Stumm,Gerd Meyer Zu Hörste,Jens Minnerup

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lipoprotein lipase 抗体 [LPL.A4]

Anti-Lipoprotein lipase antibody [LPL.A4]

Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (64)

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