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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Lipocalin-2 / NGAL 抗体 [EPR5084] - BSA and Azide free (ab187969)

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Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)
  • Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)
  • Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5084] to Lipocalin-2 / NGAL - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 750 APC PE Unconjugated

製品の概要

  • 製品名

    Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free
    Lipocalin-2 / NGAL 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR5084] to Lipocalin-2 / NGAL - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab187960)

  • ポジティブ･コントロール

    • Human fetal kidney lysate and SW480 cell lysate. IHC-P: human kidney tissue

  • 特記事項

    ab187969 is the carrier-free version of ab125075.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187969の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.Can be blocked with Lipocalin-2 / NGAL peptide (ab187960).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.Can be blocked with Lipocalin-2 / NGAL peptide (ab187960).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.

  • 組織特異性

    Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.

  • 配列類似性

    Belongs to the calycin superfamily. Lipocalin family.

  • 細胞内局在

    Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 24p3 antibody
      • 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
      • 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
      • Alpha 2 microglobulin related protein antibody
      • HGNC:6526 antibody
      • HNL antibody
      • Lcn 2 antibody
      • Lcn2 antibody
      • Lipocalin-2 antibody
      • Migration stimulating factor inhibitor antibody
      • MSFI antibody
      • Neutrophil gelatinase associated lipocalin antibody
      • Neutrophil gelatinase associated lipocalin precursor antibody
      • Neutrophil gelatinase-associated lipocalin antibody
      • NGAL antibody
      • NGAL_HUMAN antibody
      • Oncogene 24p3 antibody
      • Oncogenic lipocalin 24p3 antibody
      • p25 antibody
      • Siderocalin antibody
      • siderocalin LCN2 antibody
      • SV40 induced 24P3 protein antibody
      • Uterocalin antibody
      see all

    画像

    • Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)
      Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)
      All lanes : Anti-Lipocalin-2 / NGAL antibody [EPR5084] (ab125075) at 1/1000 dilution

      Lane 1 : Wild-type SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate
      Lane 2 : LCN2 knockout SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate
      Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 22 kDa
      Observed band size: 23 kDa why is the actual band size different from the predicted?



      This data was developed using the same antibody clone in a different buffer formulation (ab125075).

      Lanes 1 - 4: Merged signal (red and green). Green - ab125075 observed at 23 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

      ab125075 was shown to react with Lipocalin-2 / NGAL in wild-type SW480 cells in Western blot with loss of signal observed in LCN2 knockout cell line ab270486 (knockout cell lysate ab270509). Wild-type SW480 and LCN2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab125075 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)
      This data was developed using the same antibody clone in a different buffer formulation (ab125075).
      Immunohistochemical analysis of paraffin-embedded human kidney tissue sections labeling Lipocalin-2 / NGAL with purified ab125075 at 1/100 dilution. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Sections were counterstained with Hematoxylin.
      Antigen retrieval was heat mediated antigen retrieval using citrate buffer, pH 6.0).
    • Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)
      Western blot - Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)
      All lanes : Anti-Lipocalin-2 / NGAL antibody [EPR5084] (ab125075) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : SW480 (Human colorectal adenocarcinoma cell line) cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 22 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125075).

    • Immunocytochemistry/ Immunofluorescence - Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)
      Immunocytochemistry/ Immunofluorescence - Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)
      This data was developed using the same antibody clone in a different buffer formulation (ab125075).
      Immunocytochemistry analysis of SW480 (human colorectal adenocarcinoma epithelial cell) labeling Lipocalin-2 / NGAL with purified ab125075 at 1/250 dilution (10 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.9 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
      Negative control: PBS instead of the primary antibody.
    • Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)
      Anti-Lipocalin-2 / NGAL antibody [EPR5084] - BSA and Azide free (ab187969)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab187969 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab187969 は論文での使用が確認できていません。

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