Anti-Lipocalin-2 / NGAL 抗体 (ab63929)
Key features and details
- Rabbit polyclonal to Lipocalin-2 / NGAL
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Lipocalin-2 / NGAL antibody
Lipocalin-2 / NGAL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Lipocalin-2 / NGAL -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 3819 Lipocalin-2 Antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63929の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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| 特記事項 |
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WB
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
ターゲット情報
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機能
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
組織特異性
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
配列類似性
Belongs to the calycin superfamily. Lipocalin family. -
細胞内局在
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
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参照データベース
- Entrez Gene: 16819 Mouse
- SwissProt: P11672 Mouse
- Unigene: 9537 Mouse
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別名
- 24p3 antibody
- 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
see all
画像
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Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)All lanes : Anti-Lipocalin-2 / NGAL antibody (ab63929) at 1 µg/ml
Lane 1 : Mouse Spleen at 20 µg
Lane 2 : Recombinant Mouse Lipocalin Protein 10 ng
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking Buffer - 3% Milk
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (90)
ab63929 は 90 報の論文で使用されています。
- Yuan H et al. Madecassoside ameliorates cisplatin-induced nephrotoxicity by inhibiting activation of the mitogen activated protein kinase pathway. Environ Toxicol 38:1473-1483 (2023). PubMed: 37087747
- Jerigova V et al. Chronodisruption of the acute inflammatory response by night lighting in rats. Sci Rep 13:14109 (2023). PubMed: 37644084
- Yanagi T et al. Absence of ULK1 decreases AMPK activity in the kidney, leading to chronic kidney disease progression. Genes Cells 28:5-14 (2023). PubMed: 36318474
- An X et al. Overexpression of lipocalin 2 in PBX1-deficient decidual NK cells promotes inflammation at the maternal-fetal interface. Am J Reprod Immunol 89:e13676 (2023). PubMed: 36621850
- Chu YT et al. Hypoxia-Induced Kidney Injury in Newborn Rats. Toxics 11:N/A (2023). PubMed: 36977025