Anti-Lipocalin-2 / NGAL 抗体 [29] (ab70287)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [29] (ab70287)](https://www.abcam.co.jp/ps/products/70/ab70287/Images/ab70287-218667-anti-lipocalin-2-ngal-antibody-29-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [29] (ab70287)")
Key features and details
- Rat monoclonal [29] to Lipocalin-2 / NGAL
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG1
製品の概要
-
製品名
Anti-Lipocalin-2 / NGAL antibody [29]
Lipocalin-2 / NGAL 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [29] to Lipocalin-2 / NGAL -
由来種
Rat -
特異性
ab70287 binds Lipocalin-2 / NGAL in mouse serum. -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
ab70287 is protein A/G purified. -
ポリ/モノ
モノクローナル -
クローン名
29 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70287の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration.
Microwave pretreatment using citrate buffer pH 6, is recommended. |
| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. Microwave pretreatment using citrate buffer pH 6, is recommended. |
ターゲット情報
-
機能
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
組織特異性
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
配列類似性
Belongs to the calycin superfamily. Lipocalin family. -
細胞内局在
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
-
参照データベース
- Entrez Gene: 16819 Mouse
- SwissProt: P11672 Mouse
- Unigene: 9537 Mouse
-
別名
- 24p3 antibody
- 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [29] (ab70287)
Lipocalin-2 / NGAL expression in the ischemia-reperfusion injured mouse kidney; detected with anti-mouse Lipocalin-2 / NGAL antibody (ab70287) (1:1000).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [29] (ab70287)](https://www.abcam.co.jp/ps/products/70/ab70287/Images/ab70287-228752-anti-lipocalin-2-ngal-antibody-29-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [29] (ab70287)Lipocalin-2 / NGAL expression 2 days after the introduction of ischemic-reperfusion injured mouse kidney; detected with ab70287 at 1/1000.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (9)
ab70287 は 9 報の論文で使用されています。
- Omachi K et al. PPAR δ Agonism Ameliorates Renal Fibrosis in an Alport Syndrome Mouse Model. Kidney360 4:341-348 (2023). PubMed: 36657027
- Raghavan R et al. Plakophilin3 loss leads to increased adenoma formation and rectal prolapse in APCmin mice. Biochem Biophys Res Commun 586:14-19 (2022). PubMed: 34823217
- Jo J et al. Protective Effects of Orexin A in a Murine Model of Cisplatin-Induced Acute Kidney Injury. J Clin Med 11:N/A (2022). PubMed: 36498769
- Li H et al. Tubular β-catenin alleviates mitochondrial dysfunction and cell death in acute kidney injury. Cell Death Dis 13:1061 (2022). PubMed: 36539406
- Chiba T et al. Endothelial-Derived miR-17∼92 Promotes Angiogenesis to Protect against Renal Ischemia-Reperfusion Injury. J Am Soc Nephrol 32:553-562 (2021). PubMed: 33514560
- Kholia S et al. Human Liver Stem Cell Derived Extracellular Vesicles Alleviate Kidney Fibrosis by Interfering with the β-Catenin Pathway through miR29b. Int J Mol Sci 22:N/A (2021). PubMed: 34639119
- Ni Y et al. Interruption of neutrophil extracellular traps formation dictates host defense and tubular HOXA5 stability to augment efficacy of anti-Fn14 therapy against septic AKI. Theranostics 11:9431-9451 (2021). PubMed: 34646379
- Shim YJ et al. Early T cell infiltration is modulated by programed cell death-1 protein and its ligand (PD-1/PD-L1) interactions in murine kidney transplants. Kidney Int 98:897-905 (2020). PubMed: 32763116
- Chiba T et al. Sirtuin 5 Regulates Proximal Tubule Fatty Acid Oxidation to Protect against AKI. J Am Soc Nephrol 30:2384-2398 (2019). PubMed: 31575700