Anti-Lingo1 抗体 (ab23631)
Key features and details
- Rabbit polyclonal to Lingo1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Lingo1 antibody
Lingo1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Lingo1 -
由来種
Rabbit -
特異性
Rabbit polyclonal to Lingo1 (ab23631) detects Lingo1 protein at ~83kDa in mouse and human brain lysates. This band is larger than predicted on Swiss Prot (69kDa; Q9D1T0) possibly due to post-translational modification and is consistent with published literature on Lingo1 protein detection in brain lysate. The strong band observed at ~ 17kDa in the mouse brain lysate (lane 1) corresponds to a cleavage fragment of Lingo1 (Swiss Prot IDs: Q3TQJ4) -
アプリケーション
適用あり: WB, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken
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免疫原
Synthetic peptide corresponding to Human Lingo1 aa 600 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab25890) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23631の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| IP |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
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ICC/IF
Use a concentration of 5 µg/ml. |
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IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. Is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. Acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development. -
組織特異性
Expressed exclusively in the central nervous system. Highest level in the in amygdala, hippocampus, thalamus and cerebral cortex. In the rest of the brain a basal expression seems to be always present. Up-regulated in substantia nigra neurons from Parkinson disease patients. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
翻訳後修飾
N-glycosylated. Contains predominantly high-mannose glycans. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 415344 Chicken
- Entrez Gene: 84894 Human
- Entrez Gene: 235402 Mouse
- Entrez Gene: 315691 Rat
- Omim: 609791 Human
- SwissProt: Q50L44 Chicken
- SwissProt: Q96FE5 Human
- SwissProt: Q9D1T0 Mouse
see all -
別名
- FLJ14594 antibody
- LERN 1 antibody
- LERN1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Lingo1 antibody (ab23631)
ab23631 staining Lingo1 in Ms Hippocampal Neurons E18 DIV14 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab23631 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-Lingo1 antibody (ab23631)All lanes : Anti-Lingo1 antibody (ab23631) at 1 µg/ml
Lane 1 : Mouse brain
Lane 2 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 3 : Human Brain Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Additional bands at: 17 kDa (possible cleavage fragment), 34 kDa (possible degradation product)Rabbit polyclonal to Lingo1 (ab23631) detects Lingo1 protein at ~83kDa in mouse and human brain lysates. This band is larger than predicted on Swiss Prot (69kDa; Q9D1T0) possibly due to post-translational modifications and is consistent with published literature on Lingo1 protein detection in brain lysate. The strong band observed at ~ 17kDa in the mouse brain lysate (lane 1) corresponds to a cleavage fragment of Lingo1 (Swiss Prot IDs: Q3TQJ4)
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Immunoprecipitation - Anti-Lingo1 antibody (ab23631)Lingo1 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Lingo1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab23631.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 83kDa:Lingo1; non specific - 70kDa: We are unsure as to the identity of this extra band.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab23631 は 22 報の論文で使用されています。
- Yang H et al. Anti-LINGO-1 antibody treatment alleviates cognitive deficits and promotes maturation of oligodendrocytes in the hippocampus of APP/PS1 mice. J Comp Neurol 530:1606-1621 (2022). PubMed: 35014704
- Hu H et al. Effect of ganglioside combined with Chip Jiaji electro-acupuncture on Nogo-NgR signal pathway in SCI rats. Saudi J Biol Sci 28:4132-4136 (2021). PubMed: 34354392
- Li X et al. The Role and Mechanism of AMIGO3 in the Formation of Aberrant Neural Circuits After Status Convulsion in Immature Mice. Front Mol Neurosci 14:748115 (2021). PubMed: 34650403
- Zhu J et al. LINGO-1 shRNA protects the brain against ischemia/reperfusion injury by inhibiting the activation of NF-?B and JAK2/STAT3. Hum Cell 34:1114-1122 (2021). PubMed: 33830473
- Wang YY et al. Nogo-A aggravates oxidative damage in oligodendrocytes. Neural Regen Res 16:179-185 (2021). PubMed: 32788474