Anti-LIN37 抗体 (ab184973)
Key features and details
- Rabbit polyclonal to LIN37
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-LIN37 antibody -
製品の詳細
Rabbit polyclonal to LIN37 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Recombinant fragment corresponding to Human LIN37 aa 150 to the C-terminus.
Database link: Q96GY3 -
ポジティブ･コントロール
- WB: HDLM-2 cell line; IHC-P: Human prostate, cerebellum and colon tissue; ICC: U-251 MG whole cellls.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184973の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 534550 Cow
- Entrez Gene: 55957 Human
- Entrez Gene: 75660 Mouse
- Entrez Gene: 292787 Rat
- SwissProt: Q1RMQ5 Cow
- SwissProt: Q96GY3 Human
- SwissProt: Q9D8N6 Mouse
- Unigene: 529100 Human
see all -
別名
- 1810054G18Rik antibody
- Antolefinin antibody
- Antolefinine antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIN37 antibody (ab184973)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling LIN37 with ab184973 at a 1/20 dilution.
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Western blot - Anti-LIN37 antibody (ab184973)Anti-LIN37 antibody (ab184973) at 0.4 µg/ml + Human cell line HDLM-2 -
Immunocytochemistry/ Immunofluorescence - Anti-LIN37 antibody (ab184973)Immunofluorescence analysis of U-251 MG (human brain glioma cell line) whole cellls labeling LIN37 in the nucleoplasm and mitochondria with ab184973 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIN37 antibody (ab184973)Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling LIN37 with ab184973 at a 1/20 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIN37 antibody (ab184973)Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling LIN37 with ab184973 at a 1/20 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab184973 は論文での使用が確認できていません。