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AB262859

Anti-Lin28B 抗体

Anti-Lin28B antibody

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Rabbit Polyclonal Lin28B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LIN28B aa 200 to C-terminus.

別名を表示する

CSDD2, LIN28B, Protein lin-28 homolog B, Lin-28B

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)

Paraffin-embedded Human testis tissue stained for Lin28B using ab262859 at 1/2500 dilution in immunohistochemical analysis. No expression shown as expected.

Immunocytochemistry/ Immunofluorescence - Anti-Lin28B antibody (AB262859)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Lin28B antibody (AB262859)

PFA-fixed, Triton X-100 permeabilized human HEK-293 cells stained for Lin28B (green) using ab262859 at 4 μg/ml in ICC/IF. Localization to Nucleus, nucleoli and cytosol. Cytoskeleton counterstained in Red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)

Paraffin-embedded Human liver tissue stained for Lin28B using ab262859 at 1/2500 dilution in immunohistochemical analysis. No expression shown as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)

Paraffin-embedded Human placenta tissue stained for Lin28B using ab262859 at 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (AB262859)

Paraffin-embedded Human prostate tissue stained for Lin28B using ab262859 at 1/2500 dilution in immunohistochemical analysis. Low expression shown as expected.

Western blot - Anti-Lin28B antibody (AB262859)
  • WB

Unknown

Western blot - Anti-Lin28B antibody (AB262859)

All lanes:

Western blot - Anti-Lin28B antibody (ab262859) at 0.4 µg/mL

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 27 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human LIN28B aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6ZN17

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed : 22118463). Does not act on pri-miR21 (PubMed : 22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed : 19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed : 22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
See full target information LIN28B
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代替製品

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  • 0
  • 0

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Abcam product promise

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