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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-LIMK2 抗体 [EP969Y] - C-terminal (ab45165)

Reviews (2)Q&A (1)References (9)
Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
  • Immunocytochemistry/ Immunofluorescence - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
  • Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
  • Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP969Y] to LIMK2 - C-terminal
  • Suitable for: IHC-P, ICC/IF, WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free PE

製品の概要

  • 製品名

    Anti-LIMK2 antibody [EP969Y] - C-terminal
    LIMK2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP969Y] to LIMK2 - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, ICC/IF, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Wild-type HAP1 cell lysate. Jurkat cell lysate. Ramos membrane lysate. IHC-P: Human endometrium carcinoma tissue. ICC/IF: Ramos cells.

  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab45165の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
WB (1)
1/2000. Predicted molecular weight: 72 kDa.
特記事項
IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
WB
1/2000. Predicted molecular weight: 72 kDa.

ターゲット情報

  • 機能

    Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.

  • 組織特異性

    Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.

  • 配列類似性

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Phosphorylated on serine and/or threonine residues by ROCK1.

  • 細胞内局在

    Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • LIM domain kinase 2 antibody
    • LIM kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

画像

  • Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
    Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)

    Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
    Lane 2: LIMK2 (LIMK2) knockout HAP1 whole cell lysate (40 µg)
    Lane 3: Jurkat whole cell lysate (40 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab45165 observed at 72 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab45165 was shown to specifically recognize LIMK2 (LIMK2) in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed with knockout cells were examined. Wild-type and LIMK2 (LIMK2) knockout samples were subjected to SDS-PAGE. Ab45165 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium cancer tissue labeling LIMK2 with ab45165 at 1/250 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
    Immunocytochemistry/ Immunofluorescence - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)

    Immunocytochemistry/ Immunofluorescence analysis of Ramos cells labeling LIMK2 with ab45165 at 1/250. Goat anti rabbit IgG(Alexa Fluor® 488); ab150077 at 1/1000 dilution was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI (blue) was used as a nuclear counterstain.

     

  • Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
    Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
    Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165) at 1/2000 dilution + Ramos (Human Burkitt's lymphoma cell line) membrane lysate

    Predicted band size: 72 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?

  • Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
    Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (9)

ab45165 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab45165 は 9 報の論文で使用されています。

  • Swiatlowska P  et al. Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching. Sci Adv 8:eabm3471 (2022). PubMed: 35427166
  • Tong C  et al. Tandem Mass Tag-Based Quantitative Proteomic Analysis Reveals Pathways Involved in Brain Injury Induced by Chest Exposure to Shock Waves. Front Mol Neurosci 14:688050 (2021). PubMed: 34630032
  • Yang W  et al. Co-overexpression of VEGF and GDNF in adipose-derived stem cells optimizes therapeutic effect in neurogenic erectile dysfunction model. Cell Prolif 53:e12756 (2020). PubMed: 31943490
  • Duan X  et al. The small GTPase RhoA regulates the LIMK1/2-cofilin pathway to modulate cytoskeletal dynamics in oocyte meiosis. J Cell Physiol 233:6088-6097 (2018). PubMed: 29319181
  • Hyun HW  et al. Mitochondrial Translocation of High Mobility Group Box 1 Facilitates LIM Kinase 2-Mediated Programmed Necrotic Neuronal Death. Front Cell Neurosci 10:99 (2016). PubMed: 27147971
  • Ko AR  et al. Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity. Mol Brain 8:58 (2015). IHC-FoFr ; Rat . PubMed: 26438559
  • Ray S  et al. LIM kinase regulation of cytoskeletal dynamics is required for salivary gland branching morphogenesis. Mol Biol Cell 25:2393-407 (2014). PubMed: 24966172
  • Gamell C  et al. LIMK2 mediates resistance to chemotherapeutic drugs in neuroblastoma cells through regulation of drug-induced cell cycle arrest. PLoS One 8:e72850 (2013). WB . PubMed: 23991158
  • Rice DS  et al. Keratinocyte migration in the developing eyelid requires LIMK2. PLoS One 7:e47168 (2012). IHC-P ; Mouse . PubMed: 23071748

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LIMK2 antibody [EP969Y] - C-terminal

 Poor
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9.0 @ 98°C for 30’
Permeabilization
No
Specification
spleen
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 0.4% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. GAIA MORELLO

Verified customer

投稿 Mar 19 2021

Western blot abreview for Anti-LIMK2 antibody [EP969Y] - C-terminal

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP (prostate cancer cell line))
Gel Running Conditions
Reduced Denaturing (7% tris-acetate gel)
Loading amount
10 µg
Specification
LNCaP (prostate cancer cell line)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 01 2008

Question

what's the concentration of lot 1369707?

Read More
Answer

Es freut mich Ihnen mitteilen zu können, dass wir die Konzentration des ab45165 Anti-LIM kinase 2 antibody [EP969Y] doch bestimmt haben: Für Ihr Lot beträgt sie 0.0390 mg/ml.

The concentration is 0.0390 mg/ml.

Read More

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