Anti-LIMD1 抗体 (ab114104)
Key features and details
- Rabbit polyclonal to LIMD1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-LIMD1 antibody
LIMD1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LIMD1 -
由来種
Rabbit -
アプリケーション
適用あり: IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Platypus
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免疫原
Synthetic peptide, corresponding to a region between amino acids 1-50 of Human LIMD1 (NP_055055.1).
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ポジティブ･コントロール
- Whole cell lysate from HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab114104 was affinity purified using an epitope specific to LIMD1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab114104の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Transcriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the zyxin/ajuba family.
Contains 3 LIM zinc-binding domains. -
細胞内局在
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus but is localized predominantly to the cytoplasm. Found in the nucleus but not nucleoli. - Information by UniProt
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参照データベース
- Entrez Gene: 617525 Cow
- Entrez Gene: 100856566 Dog
- Entrez Gene: 8994 Human
- Entrez Gene: 29806 Mouse
- Entrez Gene: 100738579 Pig
- Omim: 604543 Human
- SwissProt: G5E5X0 Cow
- SwissProt: Q9UGP4 Human
see all -
別名
- AW822033 antibody
- D9Ertd192e antibody
- LIM domain-containing protein 1 antibody
see all
画像
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Immunoprecipitation - Anti-LIMD1 antibody (ab114104)Detection of Human LIMD1 by Western blot of Immunoprecipitate.
LIMD1 was immunoprecipitated from HeLa whole cell lysate, using ab114104 at 6 µg/mg lysate (1 mg for IP, 20% of IP loaded). An affinity purified rabbit anti-LIMD1 antibody was used for the subsequent WB at 0.4 µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab114104 は論文での使用が確認できていません。