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Anti-LIM Kinase 1 (phospho T508) 抗体 (ab38508)

Reviews (2)Q&A (2)References (16)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)
  • Western blot - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)

Key features and details

  • Rabbit polyclonal to LIM Kinase 1 (phospho T508)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-LIM Kinase 1 (phospho T508) antibody
    LIM Kinase 1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to LIM Kinase 1 (phospho T508)

  • 由来種

    Rabbit

  • 特異性

    This antibody is specific for LIM Kinase only when phosphorylated at threonine 508. It recognizes both LIMK1 and LIMK2 from human, mouse, and rat.

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human LIM Kinase 1 (phospho T508).

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab38508の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 73 kDa).
IHC-P
1/50 - 1/100.
特記事項
WB
1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 73 kDa).
IHC-P
1/50 - 1/100.

ターゲット情報

  • 機能

    Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.

  • 組織特異性

    Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

  • 関連疾患

    Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.

  • 配列類似性

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Autophosphorylated.
    Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.

  • 細胞内局在

    Cytoplasm. Cell projection > growth cone.
  • Information by UniProt

  • 参照データベース

    • 別名

      • EC 2.7.1.37 antibody
      • LIM domain containing protein kinase antibody
      • LIM domain kinase 1 antibody
      • LIM kinase antibody
      • LIM motif containing protein kinase antibody
      • LIMK 1 antibody
      • LIMK antibody
      • LIMK-1 antibody
      • limk1 antibody
      • LIMK1_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)
      ab38508 at a 1:50 dilution staining LIM Kinase in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.

      Left image : untreated.

      Right image : treated with phosphopeptide.

    • Western blot - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)
      Western blot - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)
      All lanes : Anti-LIM Kinase 1 (phospho T508) antibody (ab38508) at 1/500 dilution

      Lane 1 : Extract of COLO cells + peptide.
      Lane 2 : Extract of COLO cells.
      Lane 3 : Extract of MCF7 cells.

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)

      Predicted band size: 73 kDa
      Observed band size: 65 kDa why is the actual band size different from the predicted?



      Lanes can be loaded with 5-30µg of total protein. The arrow next to the image points to LIM Kinase (phospho T508).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)Image from Mokalled MH et al, Development. 2010 Jul;137(14):2365-74. Epub 2010 Jun 9, Fig 6. DOI: 10.1242/dev.047605.
      ab38508 staining LIM Kinase 1 in mouse brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
      bdKO mice were anesthetized and transcardially perfused with PBS, followed by 4% paraformaldehyde (PFA) prior to brain dissection. Brains were then postfixed in 4% PFA for 2 days, embedded in paraffin and sectioned.

    参考文献 (16)

    ab38508 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab38508 は 16 報の論文で使用されています。

    • Zhu YT  et al. Environmental enrichment combined with fasudil promotes motor function recovery and axonal regeneration after stroke. Neural Regen Res 16:2512-2520 (2021). PubMed: 33907042
    • Lee KY  et al. Infantile spasms-linked Nedd4-2 mediates hippocampal plasticity and learning via cofilin signaling. EMBO Rep 22:e52645 (2021). PubMed: 34342389
    • Baharani A  et al. A Progressive Loss of phosphoSer138-Profilin Aligns with Symptomatic Course in the R6/2 Mouse Model of Huntington's Disease: Possible Sex-Dependent Signaling. Cell Mol Neurobiol N/A:N/A (2020). PubMed: 33108594
    • Ravindran S  et al. BDNF Induced Translation of Limk1 in Developing Neurons Regulates Dendrite Growth by Fine-Tuning Cofilin1 Activity. Front Mol Neurosci 12:64 (2019). PubMed: 30949027
    • Lee M  et al. Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled. J Cell Biol 218:2659-2676 (2019). PubMed: 31270137
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-LIM Kinase 1 (phospho T508) antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cultured Cells (HMC)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4-20% gradient)
    Loading amount
    40 µg
    Specification
    HMC
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 27 2019

    Western blot abreview for Anti-LIM Kinase 1 (phospho T508) antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Brain)
    Loading amount
    20 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 27 2011

    Question

    What method of antigen retrieval should I use for these two antibodies in immunohistochemistry or paraffin embedded sections?

    Read More
    Answer

    Thank you for your patience. I have been in contact with the originator of this antibody, and below is the detailed antigen retrieval protocol. The method of antigen retrieval was used for IHC-P as following: Deparaffinization 1. Incubate slide at 60? for 60 minutes. 2. Deparaffinize in Xylene for 10 minutes and repeat one more times. 3. Hydrate in 100% alcohol for 5 minutes, in 95% alcohol for 5 minutes, in 85% alcohol for 5 minutes, in 75% alcohol for 5 minutes. 4. Dip into Distill Water for 5 minutes. 5. Dip into TBS (50 mM Tris, 100 mM NaCl, pH 7.6), leave for 5 minutes, and repeat two times. Antigen Retrieval 6. Bring 500 - 2000 ml 10 mM citrate buffer (pH6.0) to the boil in a stainless steel pressure cooker. 7. Put the slide into staining rack and lower into pressure cooker ensuring the slide is well immersed in citrate buffer. 8. When the pressure indicator valve has risen after 3-4 minutes, incubate for 1 minute. 9. Cool the slide naturally to room temperature. 10. Dip into distilled water, leave for 5 minutes, and repeat two times. 11. Dip the slide in TBS for 5 minutes and repeat two times. 12. Immerse slides in 3% H2O2 (in fresh methanol) for 15 minutes at room temperature. 13. Wash with distilled water two times, 5 minutes each time. 14. Wash with TBS (pH 7.6) two times, 5 minutes each time. Staining with Primary Antibody 15. Dilute primary antibody with 3% BSA in TBS. Cover the tissue section on the slide with diluted primary antibody (use 50 – 150µl for each slide). 16. Incubate at 37? for 30 minutes or at room temperature for 60 minutes (The optimal incubation time, incubation temperature, and antibody dilution should be determined by the individual laboratory). 17. Wash with TBS two times, 5 minutes each time. Staining with Secondary Antibody 18. Incubate with 100-200µl Polymer Enhancer. Incubate 30 minutes at 37?. 19. Wash with TBS for 3 times, 5 minutes each time. 20. Incubate with 100-200µl Polymerized HRP and incubate 30 minutes at 37? 21. Wash with TBS for 3 times, 5 minutes each time. 22. Add DAB solution and incubate 3-10 minutes(The reaction progress and the optimal time should be determine according to microscope). 23. Wash with distilled water for 2 times, 5 minutes each time. 24. Counterstain sections in hematoxylin if required,wash with distilled water.Immerse slides in 0.1% HClethanol for 1-10 seconds, wash with distilled water. 25. Dehydrate through 95% ethanol for 1 minute, 100% ethanol for 2×3min, Xylene for 2×3min, and coverslip with mounting medium. I hope that this information helps and please do not hesitate to contact us for more information or advice in the future.

    Read More

    Question

    Does this recognize human phospoho-LIMK1 AND phospho-LIMK2?

    Read More
    Answer

    Thank you for your enquiry. From data of recent testing, this antibody recognizes both LIMK1 and LIMK2 from human, mouse, and rat. I have clarified this on the datasheet; should you have further questions please don't hesitate to contact me,

    Read More

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